Dynamic, mating-induced gene expression changes in female head and brain tissues of <it>Drosophila melanogaster</it>

<p>Abstract</p> <p>Background</p> <p><it>Drosophila melanogaster </it>females show changes in behavior and physiology after mating that are thought to maximize the number of progeny resulting from the most recent copulation. Sperm and seminal fluid proteins...

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Main Authors: Stirling Emma J, Sanders Laura E, Nishitani Allison, Lebo Matthew S, Knott Simon RV, Kacheria Tanvi S, Dalton Justin E, Winbush Ari, Arbeitman Michelle N
Format: Article
Language:English
Published: BMC 2010-10-01
Series:BMC Genomics
Online Access:http://www.biomedcentral.com/1471-2164/11/541
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spelling doaj-a8f548b286af40f59c20300ee5af2c152020-11-24T20:53:48ZengBMCBMC Genomics1471-21642010-10-0111154110.1186/1471-2164-11-541Dynamic, mating-induced gene expression changes in female head and brain tissues of <it>Drosophila melanogaster</it>Stirling Emma JSanders Laura ENishitani AllisonLebo Matthew SKnott Simon RVKacheria Tanvi SDalton Justin EWinbush AriArbeitman Michelle N<p>Abstract</p> <p>Background</p> <p><it>Drosophila melanogaster </it>females show changes in behavior and physiology after mating that are thought to maximize the number of progeny resulting from the most recent copulation. Sperm and seminal fluid proteins induce post-mating changes in females, however, very little is known about the resulting gene expression changes in female head and central nervous system tissues that contribute to the post-mating response.</p> <p>Results</p> <p>We determined the temporal gene expression changes in female head tissues 0-2, 24, 48 and 72 hours after mating. Females from each time point had a unique post-mating gene expression response, with 72 hours post-mating having the largest number of genes with significant changes in expression. At most time points, genes expressed in the head fat body that encode products involved in metabolism showed a marked change in expression. Additional analysis of gene expression changes in dissected brain tissues 24 hours post-mating revealed changes in transcript abundance of many genes, notably, the reduced transcript abundance of genes that encode ion channels.</p> <p>Conclusions</p> <p>Substantial changes occur in the regulation of many genes in female head tissues after mating, which might underlie aspects of the female post-mating response. These results provide new insights into the physiological and metabolic changes that accompany changes in female behaviors.</p> http://www.biomedcentral.com/1471-2164/11/541
collection DOAJ
language English
format Article
sources DOAJ
author Stirling Emma J
Sanders Laura E
Nishitani Allison
Lebo Matthew S
Knott Simon RV
Kacheria Tanvi S
Dalton Justin E
Winbush Ari
Arbeitman Michelle N
spellingShingle Stirling Emma J
Sanders Laura E
Nishitani Allison
Lebo Matthew S
Knott Simon RV
Kacheria Tanvi S
Dalton Justin E
Winbush Ari
Arbeitman Michelle N
Dynamic, mating-induced gene expression changes in female head and brain tissues of <it>Drosophila melanogaster</it>
BMC Genomics
author_facet Stirling Emma J
Sanders Laura E
Nishitani Allison
Lebo Matthew S
Knott Simon RV
Kacheria Tanvi S
Dalton Justin E
Winbush Ari
Arbeitman Michelle N
author_sort Stirling Emma J
title Dynamic, mating-induced gene expression changes in female head and brain tissues of <it>Drosophila melanogaster</it>
title_short Dynamic, mating-induced gene expression changes in female head and brain tissues of <it>Drosophila melanogaster</it>
title_full Dynamic, mating-induced gene expression changes in female head and brain tissues of <it>Drosophila melanogaster</it>
title_fullStr Dynamic, mating-induced gene expression changes in female head and brain tissues of <it>Drosophila melanogaster</it>
title_full_unstemmed Dynamic, mating-induced gene expression changes in female head and brain tissues of <it>Drosophila melanogaster</it>
title_sort dynamic, mating-induced gene expression changes in female head and brain tissues of <it>drosophila melanogaster</it>
publisher BMC
series BMC Genomics
issn 1471-2164
publishDate 2010-10-01
description <p>Abstract</p> <p>Background</p> <p><it>Drosophila melanogaster </it>females show changes in behavior and physiology after mating that are thought to maximize the number of progeny resulting from the most recent copulation. Sperm and seminal fluid proteins induce post-mating changes in females, however, very little is known about the resulting gene expression changes in female head and central nervous system tissues that contribute to the post-mating response.</p> <p>Results</p> <p>We determined the temporal gene expression changes in female head tissues 0-2, 24, 48 and 72 hours after mating. Females from each time point had a unique post-mating gene expression response, with 72 hours post-mating having the largest number of genes with significant changes in expression. At most time points, genes expressed in the head fat body that encode products involved in metabolism showed a marked change in expression. Additional analysis of gene expression changes in dissected brain tissues 24 hours post-mating revealed changes in transcript abundance of many genes, notably, the reduced transcript abundance of genes that encode ion channels.</p> <p>Conclusions</p> <p>Substantial changes occur in the regulation of many genes in female head tissues after mating, which might underlie aspects of the female post-mating response. These results provide new insights into the physiological and metabolic changes that accompany changes in female behaviors.</p>
url http://www.biomedcentral.com/1471-2164/11/541
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