Comparative gene expression profiling of placentas from patients with severe pre-eclampsia and unexplained fetal growth restriction

• Main Authors: Kurahashi Hiroki, Sekiya Takao, Kato Takema, Suzuki Machiko, Ota Sayuri, Nishizawa Haruki, Udagawa Yasuhiro
• Format: Article
• Language: English
• Published: BMC 2011-08-01
• Series: Reproductive Biology and Endocrinology
• Online Access: http://www.rbej.com/content/9/1/107

<p>Abstract</p> <p>Background</p> <p>It has been well documented that pre-eclampsia and unexplained fetal growth restriction (FGR) have a common etiological background, but little is known about their linkage at the molecular level. The aim of this study was to further investigate the mechanisms underlying pre-eclampsia and unexplained FGR.</p> <p>Methods</p> <p>We analyzed differentially expressed genes in placental tissue from severe pre-eclamptic pregnancies (<it>n </it>= 8) and normotensive pregnancies with or (<it>n </it>= 8) without FGR (<it>n </it>= 8) using a microarray method.</p> <p>Results</p> <p>A subset of the FGR samples showed a high correlation coefficient overall in the microarray data from the pre-eclampsia samples. Many genes that are known to be up-regulated in pre-eclampsia are also up-regulated in FGR, including the anti-angiogenic factors, <it>FLT1 </it>and <it>ENG</it>, believed to be associated with the onset of maternal symptoms of pre-eclampsia. A total of 62 genes were found to be differentially expressed in both disorders. However, gene set enrichment analysis for these differentially expressed genes further revealed higher expression of TP53-downstream genes in pre-eclampsia compared with FGR. TP53-downstream apoptosis-related genes, such as <it>BCL6 </it>and <it>BAX</it>, were found to be significantly more up-regulated in pre-eclampsia than in FGR, although the caspases are expressed at equivalent levels.</p> <p>Conclusions</p> <p>Our current data indicate a common pathophysiology for FGR and pre-eclampsia, leading to an up-regulation of placental anti-angiogenic factors. However, our findings also suggest that it may possibly be the excretion of these factors into the maternal circulation through the TP53-mediated early-stage apoptosis of trophoblasts that leads to the maternal symptoms of pre-eclampsia.</p>