An efficient transient expression system for gene function analysis in rose
Abstract Background Roses are widely used as garden ornamental plants and cut flowers. Rosa chinensis cv ‘Old Blush’ has been used as a model genotype in rose studies due to its contribution to recurrent flowering and tea scent traits of modern roses. The deficiency of efficient genetic transformati...
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doaj-a8ee31f243534a308c12f111b79875332020-11-25T01:04:20ZengBMCPlant Methods1746-48112017-12-0113111210.1186/s13007-017-0268-1An efficient transient expression system for gene function analysis in roseJun Lu0Mengjuan Bai1Haoran Ren2Jinyi Liu3Changquan Wang4College of Horticulture, Nanjing Agricultural UniversityCollege of Horticulture, Nanjing Agricultural UniversityCollege of Horticulture, Nanjing Agricultural UniversityCollege of Horticulture, Nanjing Agricultural UniversityCollege of Horticulture, Nanjing Agricultural UniversityAbstract Background Roses are widely used as garden ornamental plants and cut flowers. Rosa chinensis cv ‘Old Blush’ has been used as a model genotype in rose studies due to its contribution to recurrent flowering and tea scent traits of modern roses. The deficiency of efficient genetic transformation systems is a handicap limiting functional genetics studies of roses. Agrobacterium-mediated transient transformation offers a powerful tool for the characterization of gene function in plants. Results A convenient and highly efficient Agrobacterium mediated genetic transformation protocol using R. chinensis cv ‘Old Blush’ seedlings in vitro as an expression system is described in this paper. The most important factor affecting transformation efficiency in this system is seedling age; 3/4-week-old rose shoots with or without roots from sub-culturing are optimal for transformation, requiring no complicated inoculation media, supplements, or carefully tuned plant growth conditions. This transient expression system was successfully applied to analysis of the gene promoter activities, DNA binding capacity of transcription factors, protein–protein interaction in physiological contexts using luciferase as a reporter gene. Conclusions This transient transformation system was validated as a robust and efficient platform, thus providing a new option for gene function and signaling pathway investigation in roses and further extending the utility of R. chinensis cv ‘Old Blush’ as a model plant to study diverse gene function and signaling pathways in Rosaceae.http://link.springer.com/article/10.1186/s13007-017-0268-1RosesAgrobacteriumTransient transformationShoots in vitro |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jun Lu Mengjuan Bai Haoran Ren Jinyi Liu Changquan Wang |
spellingShingle |
Jun Lu Mengjuan Bai Haoran Ren Jinyi Liu Changquan Wang An efficient transient expression system for gene function analysis in rose Plant Methods Roses Agrobacterium Transient transformation Shoots in vitro |
author_facet |
Jun Lu Mengjuan Bai Haoran Ren Jinyi Liu Changquan Wang |
author_sort |
Jun Lu |
title |
An efficient transient expression system for gene function analysis in rose |
title_short |
An efficient transient expression system for gene function analysis in rose |
title_full |
An efficient transient expression system for gene function analysis in rose |
title_fullStr |
An efficient transient expression system for gene function analysis in rose |
title_full_unstemmed |
An efficient transient expression system for gene function analysis in rose |
title_sort |
efficient transient expression system for gene function analysis in rose |
publisher |
BMC |
series |
Plant Methods |
issn |
1746-4811 |
publishDate |
2017-12-01 |
description |
Abstract Background Roses are widely used as garden ornamental plants and cut flowers. Rosa chinensis cv ‘Old Blush’ has been used as a model genotype in rose studies due to its contribution to recurrent flowering and tea scent traits of modern roses. The deficiency of efficient genetic transformation systems is a handicap limiting functional genetics studies of roses. Agrobacterium-mediated transient transformation offers a powerful tool for the characterization of gene function in plants. Results A convenient and highly efficient Agrobacterium mediated genetic transformation protocol using R. chinensis cv ‘Old Blush’ seedlings in vitro as an expression system is described in this paper. The most important factor affecting transformation efficiency in this system is seedling age; 3/4-week-old rose shoots with or without roots from sub-culturing are optimal for transformation, requiring no complicated inoculation media, supplements, or carefully tuned plant growth conditions. This transient expression system was successfully applied to analysis of the gene promoter activities, DNA binding capacity of transcription factors, protein–protein interaction in physiological contexts using luciferase as a reporter gene. Conclusions This transient transformation system was validated as a robust and efficient platform, thus providing a new option for gene function and signaling pathway investigation in roses and further extending the utility of R. chinensis cv ‘Old Blush’ as a model plant to study diverse gene function and signaling pathways in Rosaceae. |
topic |
Roses Agrobacterium Transient transformation Shoots in vitro |
url |
http://link.springer.com/article/10.1186/s13007-017-0268-1 |
work_keys_str_mv |
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