An efficient transient expression system for gene function analysis in rose

Abstract Background Roses are widely used as garden ornamental plants and cut flowers. Rosa chinensis cv ‘Old Blush’ has been used as a model genotype in rose studies due to its contribution to recurrent flowering and tea scent traits of modern roses. The deficiency of efficient genetic transformati...

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Main Authors: Jun Lu, Mengjuan Bai, Haoran Ren, Jinyi Liu, Changquan Wang
Format: Article
Language:English
Published: BMC 2017-12-01
Series:Plant Methods
Subjects:
Online Access:http://link.springer.com/article/10.1186/s13007-017-0268-1
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spelling doaj-a8ee31f243534a308c12f111b79875332020-11-25T01:04:20ZengBMCPlant Methods1746-48112017-12-0113111210.1186/s13007-017-0268-1An efficient transient expression system for gene function analysis in roseJun Lu0Mengjuan Bai1Haoran Ren2Jinyi Liu3Changquan Wang4College of Horticulture, Nanjing Agricultural UniversityCollege of Horticulture, Nanjing Agricultural UniversityCollege of Horticulture, Nanjing Agricultural UniversityCollege of Horticulture, Nanjing Agricultural UniversityCollege of Horticulture, Nanjing Agricultural UniversityAbstract Background Roses are widely used as garden ornamental plants and cut flowers. Rosa chinensis cv ‘Old Blush’ has been used as a model genotype in rose studies due to its contribution to recurrent flowering and tea scent traits of modern roses. The deficiency of efficient genetic transformation systems is a handicap limiting functional genetics studies of roses. Agrobacterium-mediated transient transformation offers a powerful tool for the characterization of gene function in plants. Results A convenient and highly efficient Agrobacterium mediated genetic transformation protocol using R. chinensis cv ‘Old Blush’ seedlings in vitro as an expression system is described in this paper. The most important factor affecting transformation efficiency in this system is seedling age; 3/4-week-old rose shoots with or without roots from sub-culturing are optimal for transformation, requiring no complicated inoculation media, supplements, or carefully tuned plant growth conditions. This transient expression system was successfully applied to analysis of the gene promoter activities, DNA binding capacity of transcription factors, protein–protein interaction in physiological contexts using luciferase as a reporter gene. Conclusions This transient transformation system was validated as a robust and efficient platform, thus providing a new option for gene function and signaling pathway investigation in roses and further extending the utility of R. chinensis cv ‘Old Blush’ as a model plant to study diverse gene function and signaling pathways in Rosaceae.http://link.springer.com/article/10.1186/s13007-017-0268-1RosesAgrobacteriumTransient transformationShoots in vitro
collection DOAJ
language English
format Article
sources DOAJ
author Jun Lu
Mengjuan Bai
Haoran Ren
Jinyi Liu
Changquan Wang
spellingShingle Jun Lu
Mengjuan Bai
Haoran Ren
Jinyi Liu
Changquan Wang
An efficient transient expression system for gene function analysis in rose
Plant Methods
Roses
Agrobacterium
Transient transformation
Shoots in vitro
author_facet Jun Lu
Mengjuan Bai
Haoran Ren
Jinyi Liu
Changquan Wang
author_sort Jun Lu
title An efficient transient expression system for gene function analysis in rose
title_short An efficient transient expression system for gene function analysis in rose
title_full An efficient transient expression system for gene function analysis in rose
title_fullStr An efficient transient expression system for gene function analysis in rose
title_full_unstemmed An efficient transient expression system for gene function analysis in rose
title_sort efficient transient expression system for gene function analysis in rose
publisher BMC
series Plant Methods
issn 1746-4811
publishDate 2017-12-01
description Abstract Background Roses are widely used as garden ornamental plants and cut flowers. Rosa chinensis cv ‘Old Blush’ has been used as a model genotype in rose studies due to its contribution to recurrent flowering and tea scent traits of modern roses. The deficiency of efficient genetic transformation systems is a handicap limiting functional genetics studies of roses. Agrobacterium-mediated transient transformation offers a powerful tool for the characterization of gene function in plants. Results A convenient and highly efficient Agrobacterium mediated genetic transformation protocol using R. chinensis cv ‘Old Blush’ seedlings in vitro as an expression system is described in this paper. The most important factor affecting transformation efficiency in this system is seedling age; 3/4-week-old rose shoots with or without roots from sub-culturing are optimal for transformation, requiring no complicated inoculation media, supplements, or carefully tuned plant growth conditions. This transient expression system was successfully applied to analysis of the gene promoter activities, DNA binding capacity of transcription factors, protein–protein interaction in physiological contexts using luciferase as a reporter gene. Conclusions This transient transformation system was validated as a robust and efficient platform, thus providing a new option for gene function and signaling pathway investigation in roses and further extending the utility of R. chinensis cv ‘Old Blush’ as a model plant to study diverse gene function and signaling pathways in Rosaceae.
topic Roses
Agrobacterium
Transient transformation
Shoots in vitro
url http://link.springer.com/article/10.1186/s13007-017-0268-1
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