Melatonin enhances DNA repair capacity possibly by affecting genes involved in DNA damage responsive pathways

• Main Authors: Liu Ran, Fu Alan, Hoffman Aaron E, Zheng Tongzhang, Zhu Yong
• Format: Article
• Language: English
• Published: BMC 2013-01-01
• Series: BMC Cell Biology
• Subjects: Melatonin; DNA repair; Comet assay; Genome-wide expression; Network analysis
• Online Access: http://www.biomedcentral.com/1471-2121/14/1

<p>Abstract</p> <p>Background</p> <p>Melatonin, a hormone-like substance involved in the regulation of the circadian rhythm, has been demonstrated to protect cells against oxidative DNA damage and to inhibit tumorigenesis.</p> <p>Results</p> <p>In the current study, we investigated the effect of melatonin on DNA strand breaks using the alkaline DNA comet assay in breast cancer (MCF-7) and colon cancer (HCT-15) cell lines. Our results demonstrated that cells pretreated with melatonin had significantly shorter Olive tail moments compared to non-melatonin treated cells upon mutagen (methyl methanesulfonate, MMS) exposure, indicating an increased DNA repair capacity after melatonin treatment. We further examined the genome-wide gene expression in melatonin pretreated MCF-7 cells upon carcinogen exposure and detected altered expression of many genes involved in multiple DNA damage responsive pathways. Genes exhibiting altered expression were further analyzed for functional interrelatedness using network- and pathway-based bioinformatics analysis. The top functional network was defined as having relevance for “DNA Replication, Recombination, and Repair, Gene Expression, [and] Cancer”.</p> <p>Conclusions</p> <p>These findings suggest that melatonin may enhance DNA repair capacity by affecting several key genes involved in DNA damage responsive pathways.</p>