Antinociceptive activity of Euadenia trifoliolata (Schum. & Thonn.) Oliv. leaves and roots in mice
The leaves and roots of Euadenia trifoliolata are used in Nigeria traditional medicine for the treatment of ear ache, head ache and inflammation. The aim of the study was to evaluate the antinociceptive activity of ethanolic extract of the leaves (EL) and roots (ER) of E. trifoliolata in mice. Oral...
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doaj-a8cb2fefe34246cf9103ad5e4c605ff22020-11-25T00:29:27ZengElsevierJournal of Traditional and Complementary Medicine2225-41102016-07-016328929310.1016/j.jtcme.2015.07.006Antinociceptive activity of Euadenia trifoliolata (Schum. & Thonn.) Oliv. leaves and roots in miceMargaret O. Sofidiya0Opeyemi M. Oloruntola1Ikepo Sofola2Muyiwa S. Fageyinbo3Department of Pharmacognosy, Faculty of Pharmacy, University of Lagos, NigeriaDepartment of Pharmacognosy, Faculty of Pharmacy, University of Lagos, NigeriaDepartment of Pharmacognosy, Faculty of Pharmacy, University of Lagos, NigeriaDepartment of Pharmacology, Therapeutics and Toxicology, Faculty of Basic Medical Sciences, College of Medicine, University of Lagos, NigeriaThe leaves and roots of Euadenia trifoliolata are used in Nigeria traditional medicine for the treatment of ear ache, head ache and inflammation. The aim of the study was to evaluate the antinociceptive activity of ethanolic extract of the leaves (EL) and roots (ER) of E. trifoliolata in mice. Oral toxicity testing was performed using OECD guidelines. Antinociceptive effect was studied in mice using acetic acid-induced writhing, formalin, tail immersion and hot plate tests. Total polyphenolic contents were determined using standard methods. No mortality was recorded 24 h after oral administration of both EL and ER up to 5000 mg/kg. At the dose of 50, 100 and 200 mg/kg, administration of EL and ER resulted in significant reduction in the number of writhes compared to control. The percentage inhibition of writhings was calculated as 35.67%, 46.71% and 67.94% (EL) and 55.41%, 57.32% and 72.61% (ER), respectively. In hot plate test, EL and ER showed statistically significant antinociceptive effect, although low percentage inhibition (<50%) was recorded for ER at all the doses tested. Only EL (100 and 200 mg/kg) significantly (p < 0.001) increased the reaction time in tail immersion test. Both extracts significantly (p < 0.001) reduced the licking time in both phases of formalin test compared to control. The content of total phenolic, flavonoid and proanthocyanidin varies between the two extracts and may be the basis of the observed antinociceptive effect. The results indicate antinociceptive activity for the leaves and roots of E. trifoliolata, with the extract of the leaves showing better activity.http://www.sciencedirect.com/science/article/pii/S2225411015000826Antinociceptive activityEuadenia trifoliolataCapparaceaeLeavesRoots |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Margaret O. Sofidiya Opeyemi M. Oloruntola Ikepo Sofola Muyiwa S. Fageyinbo |
spellingShingle |
Margaret O. Sofidiya Opeyemi M. Oloruntola Ikepo Sofola Muyiwa S. Fageyinbo Antinociceptive activity of Euadenia trifoliolata (Schum. & Thonn.) Oliv. leaves and roots in mice Journal of Traditional and Complementary Medicine Antinociceptive activity Euadenia trifoliolata Capparaceae Leaves Roots |
author_facet |
Margaret O. Sofidiya Opeyemi M. Oloruntola Ikepo Sofola Muyiwa S. Fageyinbo |
author_sort |
Margaret O. Sofidiya |
title |
Antinociceptive activity of Euadenia trifoliolata (Schum. & Thonn.) Oliv. leaves and roots in mice |
title_short |
Antinociceptive activity of Euadenia trifoliolata (Schum. & Thonn.) Oliv. leaves and roots in mice |
title_full |
Antinociceptive activity of Euadenia trifoliolata (Schum. & Thonn.) Oliv. leaves and roots in mice |
title_fullStr |
Antinociceptive activity of Euadenia trifoliolata (Schum. & Thonn.) Oliv. leaves and roots in mice |
title_full_unstemmed |
Antinociceptive activity of Euadenia trifoliolata (Schum. & Thonn.) Oliv. leaves and roots in mice |
title_sort |
antinociceptive activity of euadenia trifoliolata (schum. & thonn.) oliv. leaves and roots in mice |
publisher |
Elsevier |
series |
Journal of Traditional and Complementary Medicine |
issn |
2225-4110 |
publishDate |
2016-07-01 |
description |
The leaves and roots of Euadenia trifoliolata are used in Nigeria traditional medicine for the treatment of ear ache, head ache and inflammation. The aim of the study was to evaluate the antinociceptive activity of ethanolic extract of the leaves (EL) and roots (ER) of E. trifoliolata in mice. Oral toxicity testing was performed using OECD guidelines. Antinociceptive effect was studied in mice using acetic acid-induced writhing, formalin, tail immersion and hot plate tests. Total polyphenolic contents were determined using standard methods. No mortality was recorded 24 h after oral administration of both EL and ER up to 5000 mg/kg. At the dose of 50, 100 and 200 mg/kg, administration of EL and ER resulted in significant reduction in the number of writhes compared to control. The percentage inhibition of writhings was calculated as 35.67%, 46.71% and 67.94% (EL) and 55.41%, 57.32% and 72.61% (ER), respectively. In hot plate test, EL and ER showed statistically significant antinociceptive effect, although low percentage inhibition (<50%) was recorded for ER at all the doses tested. Only EL (100 and 200 mg/kg) significantly (p < 0.001) increased the reaction time in tail immersion test. Both extracts significantly (p < 0.001) reduced the licking time in both phases of formalin test compared to control. The content of total phenolic, flavonoid and proanthocyanidin varies between the two extracts and may be the basis of the observed antinociceptive effect. The results indicate antinociceptive activity for the leaves and roots of E. trifoliolata, with the extract of the leaves showing better activity. |
topic |
Antinociceptive activity Euadenia trifoliolata Capparaceae Leaves Roots |
url |
http://www.sciencedirect.com/science/article/pii/S2225411015000826 |
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