Antioxidant Effects of Myrtus communis L.’s Essential Oils in BEAS-2B Cells Induced by Oxidative Stress with Hydrogen Peroxide

• Main Authors: Hayriye Zehra Ulutaş, Gülay Gülbol Duran
• Format: Article
• Language: English
• Published: Alanya Alaaddin Keykubat University 2020-03-01
• Series: Acta Medica Alanya
• Subjects: myrtus communis l; apopitoz; beas-2b; oksidatif stres; apoptosis; oxidative stress
• Online Access: https://dergipark.org.tr/en/pub/medalanya/issue/52507/607989?publisher=alku

Aim: In this study, the effects of Myrtus communis L. essential oil on the human bronchial epithelial cell line (BEAS-2B) exposed to oxidative stress with hydrogen peroxide were investigated and their effects on apoptotic pathways.Materials and Methods: The MTT 3- (4,5-Dimethylthiazol-2-yl) -2,5-Diphenyltetrazolium Bromide method was used to determine the appropriate doses of hydrogen peroxide (H2O2) and M. communis L.’s essential oil in BEAS-2B cells. Oxidative stress formation was determined by measuring malondialdehyde (MDA) level. The cells were divided into three groups: the group exposed to oxidative stress (group with H2O2), the treatment group (H2O2 + M. communis L.’s essential oil) and the control group. MDA levels were measured in all three groups and expression levels of Caspase 3, Caspase 8, Caspase 9 and p21 genes were determined by RT-PCR method in order to detect apoptotic effects.Results: According to MTT test results, the appropriate doses were 40 μM for H2O2 and 15.625 μg/ml for M. communis L.’s essential oil. MDA levels were significantly increased in the group treated with 40 μM H2O2 when compared with the healthy cell group (p=0.0005). In the group treated with essential oil of M. communis L., MDA level was found similar to the control group (p>0.05). Expression levels of Caspase 3, Caspase 8 and p21 genes were significantly increased in cells where H2O2 was adminisrated at 40 μM concentrations compared to healthy cell group (p=0.001, p=0.017 and p=0.0003, respectively). However, Caspase 9 gene expression level did not change significantly (p=0.8). Compared to the group in which the oxidative stress model was established, it was found that Caspase 3 gene expression level decreased significantly in the cells treated with M. communis L.’s oil (p=0.00007).Conclusion: In our study, it was shown that the essential oil of M. communis L. strongly decreased MDA levels and also had the potential to be a therapeutic agent due to its apoptotic inhibiting effect. M. communis L. has a strong antioxidant effect and is thought to be effective in stopping apoptosis caused by oxidative stress.