Microbiological reasoning of the formation and development of vulvovaginal candidiasis

• Main Authors: N. D. Yakovychuk, S. Ye. Dejneka, L. I. Sydorchuk, D. V. Rotar, I. Y. Sydorchuk, V. B. Popovych
• Format: Article
• Language: English
• Published: Zaporozhye State Medical University 2017-08-01
• Series: Zaporožskij Medicinskij Žurnal
• Subjects: candidiasis vulvovagina; Candida; lactobacillus; bifidobacterium; propianibacterium
• Online Access: http://zmj.zsmu.edu.ua/article/view/105097/101294

Aim. To conduct microbiological reasoning of the formation and development of vulvovaginal candidiasis. To study taxonomic composition and major microbiota and yeast-like fungi of genus Candida and their changes in vulvovaginal content of women with vulvovaginal candidiasis. Materials and methods. Vaginal content of 472 women (19–34 years old) with vulvovaginitis was examined. Bacteriological and mycological methods were used. Isolation of yeast-like fungi of the genus Candida was done by seeding of investigated appropriate dilutions of the material on solid nutrient Saburo medium. Species identification was carried out by microscopic, cultural properties and by enzymatic activity. Lactobacillus were isolated and grown on MRS-agar, Bifidobacterium – on modified Blaurock medium, propionic acid bacteria – yeast-serum medium. Analytical parameters characterizing the state of the microbiota of microecological biotope were defined by the commonly accepted methods. Results. The study of taxonomic composition of main microbiota of vulvovaginal content revealed that in the vulvovaginal candidiasis there is expressed deficiency of Lactobacillus and Bifidobacterium and elimination of bacteria of the genus Propionibacterium, which form the physiological basis of indigeneous protective microbiota of the biotope. It was established that C. albicans is most common (constant) in the vulvovaginal content of women with vulvovaginal candidiasis (constancy index is 76.67 %). C. zeylanoides belongs to species that are rare in vulvovaginal content, and other species of yeasts-like fungi of genus Candida (C. glabrata, C. inconspiсua, C. krusei, C. tropicalis, C. guilliermondii and C. kefyr) – the species that are rare (constancy index ranges from 1.11 to 6.30 %). Conclusions. The essential changes in the taxonomic composition of the main microbiota of vulvovaginal main content (lactobacteria, bifidobacteria and propionic acid bacteria) create the preconditions for growth and reproduction of yeast-like fungi Candida that leads to their persistence in the biotope with the subsequent pathogenic effects on the formation of vulvovaginal candidiasis. candidiasis vulvovaginal; Candida; lactobacillus; bifidobacterium; propianibacterium