Engineered yeast genomes accurately assembled from pure and mixed samples
The cost and complexity of whole genome sequencing limits its use in identifying and validating sequences used for genetic engineering and synthetic biology. Here the authors present Prymetime, an integrated workflow to sequence engineered strains and identify engineering in metagenomes.
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Publishing Group
2021-03-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-021-21656-9 |
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doaj-a84252f6c5bb479cb7fcefe72ee9704b |
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Article |
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doaj-a84252f6c5bb479cb7fcefe72ee9704b2021-03-11T11:32:29ZengNature Publishing GroupNature Communications2041-17232021-03-0112111510.1038/s41467-021-21656-9Engineered yeast genomes accurately assembled from pure and mixed samplesJoseph H. Collins0Kevin W. Keating1Trent R. Jones2Shravani Balaji3Celeste B. Marsan4Marina Çomo5Zachary J. Newlon6Tom Mitchell7Bryan Bartley8Aaron Adler9Nicholas Roehner10Eric M. Young11Department of Chemical Engineering, Worcester Polytechnic InstituteDepartment of Chemical Engineering, Worcester Polytechnic InstituteDepartment of Chemical Engineering, Worcester Polytechnic InstituteDepartment of Chemical Engineering, Worcester Polytechnic InstituteDepartment of Chemical Engineering, Worcester Polytechnic InstituteDepartment of Chemical Engineering, Worcester Polytechnic InstituteDepartment of Chemical Engineering, Worcester Polytechnic InstituteSynthetic Biology, Raytheon BBN TechnologiesSynthetic Biology, Raytheon BBN TechnologiesSynthetic Biology, Raytheon BBN TechnologiesSynthetic Biology, Raytheon BBN TechnologiesDepartment of Chemical Engineering, Worcester Polytechnic InstituteThe cost and complexity of whole genome sequencing limits its use in identifying and validating sequences used for genetic engineering and synthetic biology. Here the authors present Prymetime, an integrated workflow to sequence engineered strains and identify engineering in metagenomes.https://doi.org/10.1038/s41467-021-21656-9 |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Joseph H. Collins Kevin W. Keating Trent R. Jones Shravani Balaji Celeste B. Marsan Marina Çomo Zachary J. Newlon Tom Mitchell Bryan Bartley Aaron Adler Nicholas Roehner Eric M. Young |
| spellingShingle |
Joseph H. Collins Kevin W. Keating Trent R. Jones Shravani Balaji Celeste B. Marsan Marina Çomo Zachary J. Newlon Tom Mitchell Bryan Bartley Aaron Adler Nicholas Roehner Eric M. Young Engineered yeast genomes accurately assembled from pure and mixed samples Nature Communications |
| author_facet |
Joseph H. Collins Kevin W. Keating Trent R. Jones Shravani Balaji Celeste B. Marsan Marina Çomo Zachary J. Newlon Tom Mitchell Bryan Bartley Aaron Adler Nicholas Roehner Eric M. Young |
| author_sort |
Joseph H. Collins |
| title |
Engineered yeast genomes accurately assembled from pure and mixed samples |
| title_short |
Engineered yeast genomes accurately assembled from pure and mixed samples |
| title_full |
Engineered yeast genomes accurately assembled from pure and mixed samples |
| title_fullStr |
Engineered yeast genomes accurately assembled from pure and mixed samples |
| title_full_unstemmed |
Engineered yeast genomes accurately assembled from pure and mixed samples |
| title_sort |
engineered yeast genomes accurately assembled from pure and mixed samples |
| publisher |
Nature Publishing Group |
| series |
Nature Communications |
| issn |
2041-1723 |
| publishDate |
2021-03-01 |
| description |
The cost and complexity of whole genome sequencing limits its use in identifying and validating sequences used for genetic engineering and synthetic biology. Here the authors present Prymetime, an integrated workflow to sequence engineered strains and identify engineering in metagenomes. |
| url |
https://doi.org/10.1038/s41467-021-21656-9 |
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