Effect of cholecystokinin octapeptide on mean pulmonary artery pressure in endotoxic shock rats

• Main Authors: Dong Zhang, Bin Cong
• Format: Article
• Language: English
• Published: Editorial Board of Journal of Hainan Medical University 2020-07-01
• Series: Journal of Hainan Medical University
• Subjects: cholecystokinin; lipopolysaccharide; endotoxin shock; map; mpap
• Online Access: http://www.hnykdxxb.com/PDF/202014/05.pdf

Objective: To investigate the effects of CCK-8 on mean pulmonary artery pressure in rats with endotoxic shock. Methods: Male SD rats were randomized into seven groups (n=6): control group, model group, LPS+CCK-8 group, CCK-8 group, CCK-1R antagonist group, CCK- 2R antagonist group, DFSO+PF group. The rats were induced to lethal endotoxic shock by an injection of LPS (30 mg.kg-1). CCK-8 (50 μg. kg-1) was administered 30 min after LPS injection. Either a specific CCK-1R antagonist or CCK-2R antagonist was injected before CCK-8 treatment. The mean arterial pressure (MAP) and mean pulmonary artery pressure (MPAP) were collected by a multi-channel data physiological recorder. As well as the 8h mortality was recorded. Results: Compared with control group, the MAP were significantly continuously lower and the MPAP significantly higher in model group. Administration of CCK-8 significantly delayed the LPS-induced not only decreases in MAP but also rises in MPAP, while reduceing the mortality. In addition, the specific antagonist at the CCK-2 receptor (CCK-2R) abrogated the action of CCK-8 significantly. Conclusion: while the LPSinduced hypotension delayed, CCK-8 could effectively alleviate the LPS-induced rises in MPAP via the CCK-2 receptor in ES rat model, while reduceing the mortality.