Detection of serum human epididymis secretory protein 4 in patients with ovarian cancer using a label-free biosensor based on localized surface plasmon resonance

Jialing Yuan,1 Ruiqi Duan,1 Huan Yang,2 Xiangang Luo,2 Mingrong Xi11Department of Gynecology and Obstetrics, West China Second University Hospital, Sichuan University, 2State Key Laboratory of Optical Technologies for Microfabrication, Institute of Optics and Electronics, Chinese Academy of Science,...

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Main Authors: Yuan JL, Duan RQ, Yang H, Luo XG, Xi MR
Format: Article
Language:English
Published: Dove Medical Press 2012-06-01
Series:International Journal of Nanomedicine
Online Access:http://www.dovepress.com/detection-of-serum-human-epididymis-secretory-protein-4-in-patients-wi-a10091
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spelling doaj-a7f62f89418e44e7b4ee34200e8e7fb32020-11-25T01:05:57ZengDove Medical PressInternational Journal of Nanomedicine1176-91141178-20132012-06-012012default29212928Detection of serum human epididymis secretory protein 4 in patients with ovarian cancer using a label-free biosensor based on localized surface plasmon resonanceYuan JLDuan RQYang HLuo XGXi MRJialing Yuan,1 Ruiqi Duan,1 Huan Yang,2 Xiangang Luo,2 Mingrong Xi11Department of Gynecology and Obstetrics, West China Second University Hospital, Sichuan University, 2State Key Laboratory of Optical Technologies for Microfabrication, Institute of Optics and Electronics, Chinese Academy of Science, Chengdu, ChinaBackground: Detection of the human epididymis secretory protein 4 (HE4) biomarker plays an important role in the early diagnosis of ovarian cancer. This study aimed to develop a novel localized surface plasmon resonance (LSPR) biosensor for detecting HE4 in blood samples from patients with ovarian cancer.Methods: Silver nanoparticles were fabricated using a nanosphere lithography method. The anti-HE4 antibody as a probe, which can distinctly recognize HE4, was assembled onto the nanochip surface using an amine coupling method. Detection was based on the shift in the extinction maximum of the LSPR spectrum before and after the HE4-anti-HE4 antibody reaction. These nanobiosensors were applied to detect HE4 in human serum samples and compare them using an enzyme-linked immunosorbent assay.Results: Tests relating to the detection of HE4 demonstrated that the LSPR-based biosensor featured a fast detection speed, good specificity, effective reproducibility, and long-term stability. The linear range for LSPR was between 10 pM and 10,000 pM, with a detection limit of 4 pM. An excellent correlation between LSPR and enzyme-linked immunosorbent assay results was observed in human serum.Conclusion: This study is the first clinical diagnostic application of the LSPR biosensor in ovarian cancer. The LSPR biosensor, a rapid, low-cost, label-free and portable screening tool, can serve as a very effective alternative for the clinical serological diagnosis of ovarian cancer.Keywords: localized surface plasmon resonance system, nanobiosensor, ovarian cancer biomarker, serum HE4 proteinhttp://www.dovepress.com/detection-of-serum-human-epididymis-secretory-protein-4-in-patients-wi-a10091
collection DOAJ
language English
format Article
sources DOAJ
author Yuan JL
Duan RQ
Yang H
Luo XG
Xi MR
spellingShingle Yuan JL
Duan RQ
Yang H
Luo XG
Xi MR
Detection of serum human epididymis secretory protein 4 in patients with ovarian cancer using a label-free biosensor based on localized surface plasmon resonance
International Journal of Nanomedicine
author_facet Yuan JL
Duan RQ
Yang H
Luo XG
Xi MR
author_sort Yuan JL
title Detection of serum human epididymis secretory protein 4 in patients with ovarian cancer using a label-free biosensor based on localized surface plasmon resonance
title_short Detection of serum human epididymis secretory protein 4 in patients with ovarian cancer using a label-free biosensor based on localized surface plasmon resonance
title_full Detection of serum human epididymis secretory protein 4 in patients with ovarian cancer using a label-free biosensor based on localized surface plasmon resonance
title_fullStr Detection of serum human epididymis secretory protein 4 in patients with ovarian cancer using a label-free biosensor based on localized surface plasmon resonance
title_full_unstemmed Detection of serum human epididymis secretory protein 4 in patients with ovarian cancer using a label-free biosensor based on localized surface plasmon resonance
title_sort detection of serum human epididymis secretory protein 4 in patients with ovarian cancer using a label-free biosensor based on localized surface plasmon resonance
publisher Dove Medical Press
series International Journal of Nanomedicine
issn 1176-9114
1178-2013
publishDate 2012-06-01
description Jialing Yuan,1 Ruiqi Duan,1 Huan Yang,2 Xiangang Luo,2 Mingrong Xi11Department of Gynecology and Obstetrics, West China Second University Hospital, Sichuan University, 2State Key Laboratory of Optical Technologies for Microfabrication, Institute of Optics and Electronics, Chinese Academy of Science, Chengdu, ChinaBackground: Detection of the human epididymis secretory protein 4 (HE4) biomarker plays an important role in the early diagnosis of ovarian cancer. This study aimed to develop a novel localized surface plasmon resonance (LSPR) biosensor for detecting HE4 in blood samples from patients with ovarian cancer.Methods: Silver nanoparticles were fabricated using a nanosphere lithography method. The anti-HE4 antibody as a probe, which can distinctly recognize HE4, was assembled onto the nanochip surface using an amine coupling method. Detection was based on the shift in the extinction maximum of the LSPR spectrum before and after the HE4-anti-HE4 antibody reaction. These nanobiosensors were applied to detect HE4 in human serum samples and compare them using an enzyme-linked immunosorbent assay.Results: Tests relating to the detection of HE4 demonstrated that the LSPR-based biosensor featured a fast detection speed, good specificity, effective reproducibility, and long-term stability. The linear range for LSPR was between 10 pM and 10,000 pM, with a detection limit of 4 pM. An excellent correlation between LSPR and enzyme-linked immunosorbent assay results was observed in human serum.Conclusion: This study is the first clinical diagnostic application of the LSPR biosensor in ovarian cancer. The LSPR biosensor, a rapid, low-cost, label-free and portable screening tool, can serve as a very effective alternative for the clinical serological diagnosis of ovarian cancer.Keywords: localized surface plasmon resonance system, nanobiosensor, ovarian cancer biomarker, serum HE4 protein
url http://www.dovepress.com/detection-of-serum-human-epididymis-secretory-protein-4-in-patients-wi-a10091
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