CRISPR Activation Enhances In Vitro Potency of AAV Vectors Driven by Tissue-Specific Promoters

Validation of gene transfer vectors containing tissue-specific promoters in cell-based functional assays poses a formidable challenge for gene therapy product development. Here, we describe a novel approach based on CRISPR/dCas9 transcriptional activation to achieve robust transgene expression from...

Full description

Bibliographic Details
Main Authors: Devin S. McDougald, Thu T. Duong, Katherine C. Palozola, Anson Marsh, Tyler E. Papp, Jason A. Mills, Shangzhen Zhou, Jean Bennett
Format: Article
Language:English
Published: Elsevier 2019-06-01
Series:Molecular Therapy: Methods & Clinical Development
Online Access:http://www.sciencedirect.com/science/article/pii/S2329050119300312

Similar Items