| Summary: | The lipid composition of normal Swiss laboratory mouse liver has been determined by chromatography and infrared spectrophotometry. The neutral lipids were mainly triglycerides (80%) with cholesterol, cholesterol ester, and an unidentified hydrocarbon also present. No free fatty acids were isolated. The phospholipids were largely lecithin (58%), with phosphatidyl ethanolamine, phosphatidyl serine, monophosphoinositol, sphingomyelin, and cardiolipin also present. A trace of lysolecithin was also detected, although it was impossible to determine whether or not it was an artifact of the isolation procedure.The fatty acid composition of the isolated lipid fractions was determined by gas-liquid chromatography. The neutral lipids contained mainly palmitic, oleic, and linoleic acid, while the phospholipids contained large quantities of C20 and C22 polyunsaturated acids and considerably more stearic acid than the neutral lipids. The cardiolipin fraction had a high content of linoleic acid.
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