Screening, Isolation and Identification of Thermophilic Esterase Enzyme Isolated from Rhodococcus SP: LKE-021
Rhodococcus sp. LKE-021 from soil samples of the region of Gangotri (10,000 feet of average height) of Uttarakhand Himalayas, India, produced a thermophilic esterase. The physiological and morphological characteristics of the isolated Rhodococcus sp. LKE-021 detected as Gram Positive, rod shape, cat...
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Journal of Pure and Applied Microbiology
2019-09-01
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| Series: | Journal of Pure and Applied Microbiology |
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| Online Access: | https://microbiologyjournal.org/screening-isolation-and-identification-of-thermophilic-esterase-enzyme-isolated-from-rhodococcus-sp-lke-021/ |
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doaj-a767feca20ef42879739da6946b243692021-10-02T17:24:11ZengJournal of Pure and Applied MicrobiologyJournal of Pure and Applied Microbiology0973-75102581-690X2019-09-011331855186110.22207/JPAM.13.3.63Screening, Isolation and Identification of Thermophilic Esterase Enzyme Isolated from Rhodococcus SP: LKE-021Lekha Singh0Gaurav Sharma1Gyanendra Awasthi2Lokendra Kumar3Mohammad Irfan Ali4Sarmad Moin51School of Applied Sciences, Suresh Gyan Vihar University, Jaipur - 302 033, India. 2Department of Biochemistry, Dolphin PG Institute of Biomedical and Natural Science, Dehradun - 248 007, India.1School of Applied Sciences, Suresh Gyan Vihar University, Jaipur - 302 033, India. 2Department of Biochemistry, Dolphin PG Institute of Biomedical and Natural Science, Dehradun - 248 007, India. 2Department of Biochemistry, Dolphin PG Institute of Biomedical and Natural Science, Dehradun - 248 007, India. 3Department of Microbiology, PM College of Education Karsua, Aligarh - 202 001, India.1School of Applied Sciences, Suresh Gyan Vihar University, Jaipur - 302 033, India. 1School of Applied Sciences, Suresh Gyan Vihar University, Jaipur - 302 033, India. Rhodococcus sp. LKE-021 from soil samples of the region of Gangotri (10,000 feet of average height) of Uttarakhand Himalayas, India, produced a thermophilic esterase. The physiological and morphological characteristics of the isolated Rhodococcus sp. LKE-021 detected as Gram Positive, rod shape, catalase positive, indole negative, positive to glucose and xylose fermentation test, and can grow on the Nutrient Broth medium. Esterase production confirmed on the basis of spectrophotometric enzyme assay. Taxonomic characteristics Rhodococcus confirmed by 16s rRNA gene sequencing. https://microbiologyjournal.org/screening-isolation-and-identification-of-thermophilic-esterase-enzyme-isolated-from-rhodococcus-sp-lke-021/esterasehydrolytic enzymesrhodococcuslke -021extremophiles enzymethermophilic enzyme |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Lekha Singh Gaurav Sharma Gyanendra Awasthi Lokendra Kumar Mohammad Irfan Ali Sarmad Moin |
| spellingShingle |
Lekha Singh Gaurav Sharma Gyanendra Awasthi Lokendra Kumar Mohammad Irfan Ali Sarmad Moin Screening, Isolation and Identification of Thermophilic Esterase Enzyme Isolated from Rhodococcus SP: LKE-021 Journal of Pure and Applied Microbiology esterase hydrolytic enzymes rhodococcus lke -021 extremophiles enzyme thermophilic enzyme |
| author_facet |
Lekha Singh Gaurav Sharma Gyanendra Awasthi Lokendra Kumar Mohammad Irfan Ali Sarmad Moin |
| author_sort |
Lekha Singh |
| title |
Screening, Isolation and Identification of Thermophilic Esterase Enzyme Isolated from Rhodococcus SP: LKE-021 |
| title_short |
Screening, Isolation and Identification of Thermophilic Esterase Enzyme Isolated from Rhodococcus SP: LKE-021 |
| title_full |
Screening, Isolation and Identification of Thermophilic Esterase Enzyme Isolated from Rhodococcus SP: LKE-021 |
| title_fullStr |
Screening, Isolation and Identification of Thermophilic Esterase Enzyme Isolated from Rhodococcus SP: LKE-021 |
| title_full_unstemmed |
Screening, Isolation and Identification of Thermophilic Esterase Enzyme Isolated from Rhodococcus SP: LKE-021 |
| title_sort |
screening, isolation and identification of thermophilic esterase enzyme isolated from rhodococcus sp: lke-021 |
| publisher |
Journal of Pure and Applied Microbiology |
| series |
Journal of Pure and Applied Microbiology |
| issn |
0973-7510 2581-690X |
| publishDate |
2019-09-01 |
| description |
Rhodococcus sp. LKE-021 from soil samples of the region of Gangotri (10,000 feet of average height) of Uttarakhand Himalayas, India, produced a thermophilic esterase. The physiological and morphological characteristics of the isolated Rhodococcus sp. LKE-021 detected as Gram Positive, rod shape, catalase positive, indole negative, positive to glucose and xylose fermentation test, and can grow on the Nutrient Broth medium. Esterase production confirmed on the basis of spectrophotometric enzyme assay. Taxonomic characteristics Rhodococcus confirmed by 16s rRNA gene sequencing.
|
| topic |
esterase hydrolytic enzymes rhodococcus lke -021 extremophiles enzyme thermophilic enzyme |
| url |
https://microbiologyjournal.org/screening-isolation-and-identification-of-thermophilic-esterase-enzyme-isolated-from-rhodococcus-sp-lke-021/ |
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