Apoptosis Induction of Lycorine on Lung Cancer NCI-H460 Cells and Related Mechanism

• Main Authors: LI Juwei, YANG Yang
• Format: Article
• Language: zho
• Published: Magazine House of Cancer Research on Prevention and Treatment 2018-01-01
• Series: Zhongliu Fangzhi Yanjiu
• Subjects: lycorine; lung cancer; nci-h460 cells; apoptosis; caspase 3
• Online Access: http://html.rhhz.net/ZLFZYJ/html/8578.2018.17.0388.htm

Objective To investigate the effect of lycorine on the proliferation inhibition and apoptosis induction in human lung cancer NCI-H460 cells, and to explore the anti-tumor mechanism of lycorine. Methods MTT assay was used to detect the proliferation of NCI-H460 cells. The apoptosis rate of NCI-H460 cells was detected by flow cytometry. The activity of Caspase 3 was detected by colorimetry. Results Lycorine could inhibit the proliferation of NCI-H460 cells, and the IC50 was (5.79±0.11)μmol/L. Lycorine had an induced effect on the apoptosis of NCI-H460 cells. Lycorine could enhance Caspase 3 activity. Conclusion Lycorine could effectively inhibit the proliferation and induce the apoptosis of NCI-H460 cells in vitro, and its mechanism may be related to the activation of Caspase 3 expression and the apoptosis induce of tumor cells.