New platform for simple and rapid protein-based affinity reactions

New platform for simple and rapid protein-based affinity reactions

Abstract We developed a spongy-like porous polymer (spongy monolith) consisting of poly(ethylene-co-glycidyl methacrylate) with continuous macropores that allowed efficient in situ reaction between the epoxy groups and proteins of interest. Immobilization of protein A on the spongy monolith enabled...

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Main Authors: Kei Kubota, Takuya Kubo, Tetsuya Tanigawa, Toyohiro Naito, Koji Otsuka
Format: Article
Language:English
Published: Nature Publishing Group 2017-03-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-017-00264-y
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spelling doaj-a7505426d50b459395c7bf62aa29c32a2020-12-08T00:50:17ZengNature Publishing GroupScientific Reports2045-23222017-03-01711910.1038/s41598-017-00264-yNew platform for simple and rapid protein-based affinity reactionsKei Kubota0Takuya Kubo1Tetsuya Tanigawa2Toyohiro Naito3Koji Otsuka4Graduate School of Engineering, Kyoto UniversityGraduate School of Engineering, Kyoto UniversityGraduate School of Engineering, Kyoto UniversityGraduate School of Engineering, Kyoto UniversityGraduate School of Engineering, Kyoto UniversityAbstract We developed a spongy-like porous polymer (spongy monolith) consisting of poly(ethylene-co-glycidyl methacrylate) with continuous macropores that allowed efficient in situ reaction between the epoxy groups and proteins of interest. Immobilization of protein A on the spongy monolith enabled high-yield collection of immunoglobulin G (IgG) from cell culture supernatant even at a high flow rate. In addition, immobilization of pepsin on the spongy monolith enabled efficient online digestion at a high flow rate.https://doi.org/10.1038/s41598-017-00264-y
collection DOAJ
language English
format Article
sources DOAJ
author Kei Kubota
Takuya Kubo
Tetsuya Tanigawa
Toyohiro Naito
Koji Otsuka
spellingShingle Kei Kubota
Takuya Kubo
Tetsuya Tanigawa
Toyohiro Naito
Koji Otsuka
New platform for simple and rapid protein-based affinity reactions
Scientific Reports
author_facet Kei Kubota
Takuya Kubo
Tetsuya Tanigawa
Toyohiro Naito
Koji Otsuka
author_sort Kei Kubota
title New platform for simple and rapid protein-based affinity reactions
title_short New platform for simple and rapid protein-based affinity reactions
title_full New platform for simple and rapid protein-based affinity reactions
title_fullStr New platform for simple and rapid protein-based affinity reactions
title_full_unstemmed New platform for simple and rapid protein-based affinity reactions
title_sort new platform for simple and rapid protein-based affinity reactions
publisher Nature Publishing Group
series Scientific Reports
issn 2045-2322
publishDate 2017-03-01
description Abstract We developed a spongy-like porous polymer (spongy monolith) consisting of poly(ethylene-co-glycidyl methacrylate) with continuous macropores that allowed efficient in situ reaction between the epoxy groups and proteins of interest. Immobilization of protein A on the spongy monolith enabled high-yield collection of immunoglobulin G (IgG) from cell culture supernatant even at a high flow rate. In addition, immobilization of pepsin on the spongy monolith enabled efficient online digestion at a high flow rate.
url https://doi.org/10.1038/s41598-017-00264-y
work_keys_str_mv AT keikubota newplatformforsimpleandrapidproteinbasedaffinityreactions
AT takuyakubo newplatformforsimpleandrapidproteinbasedaffinityreactions
AT tetsuyatanigawa newplatformforsimpleandrapidproteinbasedaffinityreactions
AT toyohironaito newplatformforsimpleandrapidproteinbasedaffinityreactions
AT kojiotsuka newplatformforsimpleandrapidproteinbasedaffinityreactions
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