<i>Lobularia maritima</i> (L.) Desv. Aerial Parts Methanolic Extract: In Vitro Screening of Biological Activity

<i>Lobularia maritima</i> (L.) Desv. is a perennial herb growing wild in the Mediterranean basin. The aim of this work was to assess the fatty acid, terpene, phytosterol, and phenolic composition of the methanolic extract and its sub-fractions using Gas Chromatography-Mass Spectrometry (...

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Main Authors: Mariangela Marrelli, Maria Pia Argentieri, Pinarosa Avato, Filomena Conforti
Format: Article
Language:English
Published: MDPI AG 2020-01-01
Series:Plants
Subjects:
Online Access:https://www.mdpi.com/2223-7747/9/1/89
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spelling doaj-a743d008c8e8413aad30ed6ed7b94c332020-11-25T01:33:22ZengMDPI AGPlants2223-77472020-01-01918910.3390/plants9010089plants9010089<i>Lobularia maritima</i> (L.) Desv. Aerial Parts Methanolic Extract: In Vitro Screening of Biological ActivityMariangela Marrelli0Maria Pia Argentieri1Pinarosa Avato2Filomena Conforti3Department of Pharmacy, Health and Nutritional Sciences, University of Calabria, I-87036 Rende, ItalyDepartment of Pharmacy-Drug Sciences, Università degli Studi di Bari Aldo Moro, I-70125 Bari, ItalyDepartment of Pharmacy-Drug Sciences, Università degli Studi di Bari Aldo Moro, I-70125 Bari, ItalyDepartment of Pharmacy, Health and Nutritional Sciences, University of Calabria, I-87036 Rende, Italy<i>Lobularia maritima</i> (L.) Desv. is a perennial herb growing wild in the Mediterranean basin. The aim of this work was to assess the fatty acid, terpene, phytosterol, and phenolic composition of the methanolic extract and its sub-fractions using Gas Chromatography-Mass Spectrometry (GC-MS), High-Performance Liquid Chromathography with Dioide-Array Detector (HPLC-DAD), High-Performance Liquid Chromathography-High Resolution Mass Spectrometry (HPLC-HRMS), and Electrospray Ionization Tandem Mass Spectrometry (ESI-MS/MS). The potential health benefits of this plant species have been investigated as well. The antioxidant activity was determined in vitro by means of 2,2-diphenyl-1-picrylhydrazyl (DPPH) and &#946;-carotene bleaching tests. The inhibitory potential towards the production of the pro-inflammatory mediator nitric oxide was verified on lipopolysaccharide (LPS)-stimulated murine macrophage RAW 264.7 cell line. A remarkable inhibitory activity was observed for the dichloromethane fraction, with an IC<sub>50</sub> value equal to 45.86 &#177; 1.05 &#956;g/mL, a significant result if compared to indomethacin and the known nitric oxide synthase inhibitor N<sup>G</sup>-nitro-L-arginine methyl ester (L-NAME), used as positive controls. Moreover, the ethyl acetate fraction proved to be effective in inhibiting pancreatic lipase, an enzyme that plays a pivotal role in the gastrointestinal digestion of dietary fat, suggesting that this species could potentially be a promising source of useful compounds for the treatment of obesity.https://www.mdpi.com/2223-7747/9/1/89antioxidant<i>lobularia</i>nitric oxideobesitypancreatic lipasesweet alyssum
collection DOAJ
language English
format Article
sources DOAJ
author Mariangela Marrelli
Maria Pia Argentieri
Pinarosa Avato
Filomena Conforti
spellingShingle Mariangela Marrelli
Maria Pia Argentieri
Pinarosa Avato
Filomena Conforti
<i>Lobularia maritima</i> (L.) Desv. Aerial Parts Methanolic Extract: In Vitro Screening of Biological Activity
Plants
antioxidant
<i>lobularia</i>
nitric oxide
obesity
pancreatic lipase
sweet alyssum
author_facet Mariangela Marrelli
Maria Pia Argentieri
Pinarosa Avato
Filomena Conforti
author_sort Mariangela Marrelli
title <i>Lobularia maritima</i> (L.) Desv. Aerial Parts Methanolic Extract: In Vitro Screening of Biological Activity
title_short <i>Lobularia maritima</i> (L.) Desv. Aerial Parts Methanolic Extract: In Vitro Screening of Biological Activity
title_full <i>Lobularia maritima</i> (L.) Desv. Aerial Parts Methanolic Extract: In Vitro Screening of Biological Activity
title_fullStr <i>Lobularia maritima</i> (L.) Desv. Aerial Parts Methanolic Extract: In Vitro Screening of Biological Activity
title_full_unstemmed <i>Lobularia maritima</i> (L.) Desv. Aerial Parts Methanolic Extract: In Vitro Screening of Biological Activity
title_sort <i>lobularia maritima</i> (l.) desv. aerial parts methanolic extract: in vitro screening of biological activity
publisher MDPI AG
series Plants
issn 2223-7747
publishDate 2020-01-01
description <i>Lobularia maritima</i> (L.) Desv. is a perennial herb growing wild in the Mediterranean basin. The aim of this work was to assess the fatty acid, terpene, phytosterol, and phenolic composition of the methanolic extract and its sub-fractions using Gas Chromatography-Mass Spectrometry (GC-MS), High-Performance Liquid Chromathography with Dioide-Array Detector (HPLC-DAD), High-Performance Liquid Chromathography-High Resolution Mass Spectrometry (HPLC-HRMS), and Electrospray Ionization Tandem Mass Spectrometry (ESI-MS/MS). The potential health benefits of this plant species have been investigated as well. The antioxidant activity was determined in vitro by means of 2,2-diphenyl-1-picrylhydrazyl (DPPH) and &#946;-carotene bleaching tests. The inhibitory potential towards the production of the pro-inflammatory mediator nitric oxide was verified on lipopolysaccharide (LPS)-stimulated murine macrophage RAW 264.7 cell line. A remarkable inhibitory activity was observed for the dichloromethane fraction, with an IC<sub>50</sub> value equal to 45.86 &#177; 1.05 &#956;g/mL, a significant result if compared to indomethacin and the known nitric oxide synthase inhibitor N<sup>G</sup>-nitro-L-arginine methyl ester (L-NAME), used as positive controls. Moreover, the ethyl acetate fraction proved to be effective in inhibiting pancreatic lipase, an enzyme that plays a pivotal role in the gastrointestinal digestion of dietary fat, suggesting that this species could potentially be a promising source of useful compounds for the treatment of obesity.
topic antioxidant
<i>lobularia</i>
nitric oxide
obesity
pancreatic lipase
sweet alyssum
url https://www.mdpi.com/2223-7747/9/1/89
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