The Effect of Genomic DNA Contamination on the Detection of Circulating Long Non-Coding RNAs: The Paradigm of <i>MALAT1</i>

The Effect of Genomic DNA Contamination on the Detection of Circulating Long Non-Coding RNAs: The Paradigm of <i>MALAT1</i>

The presence of contaminating gDNA in RNA preparations is a frequent cause of false positives in RT-PCR-based analysis. However, in some cases, this cannot be avoided, especially when there are no exons–intron junctions in the lncRNA sequences. Due to the lack of exons in few of long noncoding RNAs...

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Bibliographic Details
Main Authors: Athina N. Markou, Stavroula Smilkou, Emilia Tsaroucha, Evi Lianidou
Format: Article
Language:English
Published: MDPI AG 2021-06-01
Series:Diagnostics
Subjects:
<i>MALAT1</i>
non-long coding RNA
DNAse treatment
cfRNA
tumor biomarkers
false positive results
Online Access:https://www.mdpi.com/2075-4418/11/7/1160

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