The membrane complexome of a new Pseudomonas strain during growth on lysogeny broth medium and medium containing glucose or phenol

The membrane complexome of a new Pseudomonas strain during growth on lysogeny broth medium and medium containing glucose or phenol

In this study, we have performed a systematic analysis of Pseudomonas sp. strain phDV1 membrane protein complexes by growing the strain in lysogeny broth medium, and medium containing glucose or phenol as sole carbon sources. In order to study the membrane complexome, we developed an approach for th...

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Bibliographic Details
Main Authors: Antigoni Nikolaki, Anastasia Papadioti, Katerina Arvaniti, Eleni Kassotaki, Julian D. Langer, Georgios Tsiotis
Format: Article
Language:English
Published: Elsevier 2014-09-01
Series:EuPA Open Proteomics
Subjects:
Complexome
Membrane protein complexes
2-D native/SDS-PAGE
Mass spectrometry
Pseudomonas
Online Access:http://www.sciencedirect.com/science/article/pii/S2212968514000282
Description
Summary:In this study, we have performed a systematic analysis of Pseudomonas sp. strain phDV1 membrane protein complexes by growing the strain in lysogeny broth medium, and medium containing glucose or phenol as sole carbon sources. In order to study the membrane complexome, we developed an approach for the extraction and the analysis of the membrane protein complexes in native conditions. Our strategy involves (a) enrichment of the membrane proteome from Pseudomonas sp. strain phDV1 by two washing steps; (b) solubilization using n-dodecyl-β-maltoside; (c) a combination of BN-PAGE with Tricine-SDS-PAGE; and (d) protein identification of tryptic peptides by mass spectrometry.
ISSN:2212-9685

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