Directed evolution of human heavy chain variable domain (VH) using in vivo protein fitness filter.

Directed evolution of human heavy chain variable domain (VH) using in vivo protein fitness filter.

Human immunoglobulin heavy chain variable domains (VH) are promising scaffolds for antigen binding. However, VH is an unstable and aggregation-prone protein, hindering its use for therapeutic purposes. To evolve the VH domain, we performed in vivo protein solubility selection that linked antibiotic...

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Bibliographic Details
Main Authors: Dong-Sik Kim, Hyung-Nam Song, Hyo Jung Nam, Sung-Geun Kim, Young-Seoub Park, Jae-Chan Park, Eui-Jeon Woo, Hyung-Kwon Lim
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2014-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC4043505?pdf=render

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