H-Point Standard Addition Method for Simultaneous Determination of Eosin and Erytrosine

H-Point Standard Addition Method for Simultaneous Determination of Eosin and Erytrosine

A new, simple, sensitive and selective H-point standard addition method (HPSAM) has been developed for resolving binary mixture of food colorants eosin and erythrosine, which show overlapped spectra. The method is based on the complexation of food dyes eosin and erythrosine with Fe(III) complexing...

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Main Authors: Amandeep Kaur, Usha Gupta
Format: Article
Language:English
Published: Hindawi Limited 2011-01-01
Series:E-Journal of Chemistry
Online Access:http://dx.doi.org/10.1155/2011/210259
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spelling doaj-a6c446d188094d5bbbc45b8acd704eb02020-11-24T22:35:54ZengHindawi LimitedE-Journal of Chemistry0973-49452090-98102011-01-01841979198510.1155/2011/210259H-Point Standard Addition Method for Simultaneous Determination of Eosin and ErytrosineAmandeep Kaur0Usha Gupta1Department of Chemistry, Punjabi University, Patiala-147002, Punjab, IndiaDepartment of Chemistry, Punjabi University, Patiala-147002, Punjab, IndiaA new, simple, sensitive and selective H-point standard addition method (HPSAM) has been developed for resolving binary mixture of food colorants eosin and erythrosine, which show overlapped spectra. The method is based on the complexation of food dyes eosin and erythrosine with Fe(III) complexing reagent at pH 5.5 and solubilizing complexes in triton x-100 micellar media. Absorbances at the two pairs of wavelengths, 540 and 550 nm (when eosin acts as analyte) or 518 and 542 nm (when erythrosine act as analyte) were monitored. This method has satisfactorily been applied for the determination of eosin and erythrosine dyes in synthetic mixtures and commercial products.http://dx.doi.org/10.1155/2011/210259
collection DOAJ
language English
format Article
sources DOAJ
author Amandeep Kaur
Usha Gupta
spellingShingle Amandeep Kaur
Usha Gupta
H-Point Standard Addition Method for Simultaneous Determination of Eosin and Erytrosine
E-Journal of Chemistry
author_facet Amandeep Kaur
Usha Gupta
author_sort Amandeep Kaur
title H-Point Standard Addition Method for Simultaneous Determination of Eosin and Erytrosine
title_short H-Point Standard Addition Method for Simultaneous Determination of Eosin and Erytrosine
title_full H-Point Standard Addition Method for Simultaneous Determination of Eosin and Erytrosine
title_fullStr H-Point Standard Addition Method for Simultaneous Determination of Eosin and Erytrosine
title_full_unstemmed H-Point Standard Addition Method for Simultaneous Determination of Eosin and Erytrosine
title_sort h-point standard addition method for simultaneous determination of eosin and erytrosine
publisher Hindawi Limited
series E-Journal of Chemistry
issn 0973-4945
2090-9810
publishDate 2011-01-01
description A new, simple, sensitive and selective H-point standard addition method (HPSAM) has been developed for resolving binary mixture of food colorants eosin and erythrosine, which show overlapped spectra. The method is based on the complexation of food dyes eosin and erythrosine with Fe(III) complexing reagent at pH 5.5 and solubilizing complexes in triton x-100 micellar media. Absorbances at the two pairs of wavelengths, 540 and 550 nm (when eosin acts as analyte) or 518 and 542 nm (when erythrosine act as analyte) were monitored. This method has satisfactorily been applied for the determination of eosin and erythrosine dyes in synthetic mixtures and commercial products.
url http://dx.doi.org/10.1155/2011/210259
work_keys_str_mv AT amandeepkaur hpointstandardadditionmethodforsimultaneousdeterminationofeosinanderytrosine
AT ushagupta hpointstandardadditionmethodforsimultaneousdeterminationofeosinanderytrosine
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