Development and Evaluation of Novel and Highly Sensitive Single-Tube Nested Real-Time RT-PCR Assays for SARS-CoV-2 Detection

Development and Evaluation of Novel and Highly Sensitive Single-Tube Nested Real-Time RT-PCR Assays for SARS-CoV-2 Detection

Sensitive molecular assays are critical for coronavirus disease 2019 (COVID-19) diagnosis. Here, we designed and evaluated two single-tube nested (STN) real-time RT-PCR assays, targeting SARS-CoV-2 RdRp/Hel and <i>N</i> genes. Both STN assays had a low limit of detection and did not cros...

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Bibliographic Details
Main Authors: Cyril Chik-Yan Yip, Siddharth Sridhar, Kit-Hang Leung, Anthony Chin-Ki Ng, Kwok-Hung Chan, Jasper Fuk-Woo Chan, Owen Tak-Yin Tsang, Ivan Fan-Ngai Hung, Vincent Chi-Chung Cheng, Kwok-Yung Yuen, Kelvin Kai-Wang To
Format: Article
Language:English
Published: MDPI AG 2020-08-01
Series:International Journal of Molecular Sciences
Subjects:
SARS-CoV-2
COVID-19
single-tube nested
RT-PCR
evaluation
Online Access:https://www.mdpi.com/1422-0067/21/16/5674

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