Detection of genetic variation and activity analysis of the promoter region of the cattle tRNA-modified gene <i>TRDMT1</i>

• Main Authors: X. Yi, S. He, S. Wang, H. Zhao, M. Wu, S. Liu, X. Sun
• Format: Article
• Language: English
• Published: Copernicus Publications 2021-04-01
• Series: Archives Animal Breeding
• Online Access: https://aab.copernicus.org/articles/64/147/2021/aab-64-147-2021.pdf

<p>The tRNA modification gene in eukaryotes is relatively conservative. As an important modification gene, the <i>TRDMT1</i> gene plays an important role in maintaining tRNA structural maintenance and reducing mistranslation of protein translation by methylation of specific tRNA subpopulations. Mouse and zebrafish <i>TRDMT1</i> knockout experiments indicate that it may mediate growth and development through tRNA modification. However, there are no systematic reports on the function of tRNA-modified genes in livestock. In this study, Qinchuan cattle DNA pool sequencing technology was used. A G<span class="inline-formula">&gt;</span>C mutation in the <span class="inline-formula">−1223</span> bp position upstream of the <i>TRDMT1</i> translation initiator codon was found. At this locus, the dual-luciferase assay indicated that different genotypes cause differences in transcriptional activity (<span class="inline-formula"><i>P</i>&lt;0.05</span>). Our experiment detected a natural genetic variation of a tRNA modification gene <i>TRDMT1</i>, which may provide potential natural molecular materials for the study of tRNA modification.</p>