Detection of genetic variation and activity analysis of the promoter region of the cattle tRNA-modified gene <i>TRDMT1</i>
<p>The tRNA modification gene in eukaryotes is relatively conservative. As an important modification gene, the <i>TRDMT1</i> gene plays an important role in maintaining tRNA structural maintenance and reducing mistranslation of protein translation by methylation of specific tRNA su...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Copernicus Publications
2021-04-01
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| Series: | Archives Animal Breeding |
| Online Access: | https://aab.copernicus.org/articles/64/147/2021/aab-64-147-2021.pdf |
| Summary: | <p>The tRNA modification gene in eukaryotes is relatively
conservative. As an important modification gene, the <i>TRDMT1</i> gene plays an
important role in maintaining tRNA structural maintenance and reducing
mistranslation of protein translation by methylation of specific tRNA
subpopulations. Mouse and zebrafish <i>TRDMT1</i> knockout experiments indicate that it
may mediate growth and development through tRNA modification. However, there
are no systematic reports on the function of tRNA-modified genes in
livestock. In this study, Qinchuan cattle DNA pool sequencing technology
was used. A G<span class="inline-formula">></span>C mutation in the <span class="inline-formula">−1223</span> bp position upstream of
the <i>TRDMT1</i> translation initiator codon was found. At this locus, the dual-luciferase assay indicated that different genotypes cause differences in
transcriptional activity (<span class="inline-formula"><i>P</i><0.05</span>). Our experiment detected a natural
genetic variation of a tRNA modification gene <i>TRDMT1</i>, which may provide potential
natural molecular materials for the study of tRNA modification.</p> |
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| ISSN: | 0003-9438 2363-9822 |