Preparation, Characterization and Manipulation of Conjugates between Gold Nanoparticles and DNA

Here we described the preparation and characterization by atomic force microscopy of dumbbell-shaped conjugates between 450 bp double-stranded DNA polymer, poly(dG)-poly(dC), and 5 nm gold nanoparticles (GNPs). We have demonstrated that the size of the nanoparticles in the conjugates can be increase...

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Main Authors:	Gennady Eidelshtein, Moran Fattal, Gavriel Avishai, Benjamin Kempinski, Clelia Giannini, Alexander Kotlyar
Format:	Article
Language:	English
Published:	MDPI AG 2016-09-01
Series:	Nanomaterials
Subjects:	atomic force microscopy gold nanoparticles DNA nanotechnology poly(dG)-poly(dC)
Online Access:	http://www.mdpi.com/2079-4991/6/9/167

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spelling	doaj-a682a3e98a6e4aa49a68754e496653482020-11-24T22:48:18ZengMDPI AGNanomaterials2079-49912016-09-016916710.3390/nano6090167nano6090167Preparation, Characterization and Manipulation of Conjugates between Gold Nanoparticles and DNAGennady Eidelshtein0Moran Fattal1Gavriel Avishai2Benjamin Kempinski3Clelia Giannini4Alexander Kotlyar5Department of Biochemistry and Molecular Biology, George S. Wise Faculty of Life Sciences and the Center of Nanoscience and Nanotechnology, Tel Aviv University, Ramat Aviv, Tel Aviv 69978, IsraelDepartment of Biochemistry and Molecular Biology, George S. Wise Faculty of Life Sciences and the Center of Nanoscience and Nanotechnology, Tel Aviv University, Ramat Aviv, Tel Aviv 69978, IsraelDepartment of Biochemistry and Molecular Biology, George S. Wise Faculty of Life Sciences and the Center of Nanoscience and Nanotechnology, Tel Aviv University, Ramat Aviv, Tel Aviv 69978, IsraelDepartment of Biochemistry and Molecular Biology, George S. Wise Faculty of Life Sciences and the Center of Nanoscience and Nanotechnology, Tel Aviv University, Ramat Aviv, Tel Aviv 69978, IsraelDepartment of Chemistry, University of Milan, via Golgi 19, 20133 Milan, ItalyDepartment of Biochemistry and Molecular Biology, George S. Wise Faculty of Life Sciences and the Center of Nanoscience and Nanotechnology, Tel Aviv University, Ramat Aviv, Tel Aviv 69978, IsraelHere we described the preparation and characterization by atomic force microscopy of dumbbell-shaped conjugates between 450 bp double-stranded DNA polymer, poly(dG)-poly(dC), and 5 nm gold nanoparticles (GNPs). We have demonstrated that the size of the nanoparticles in the conjugates can be increased in a controlled fashion. Application of the conjugates for measuring the electrical conductivity of DNA is discussed.http://www.mdpi.com/2079-4991/6/9/167atomic force microscopygold nanoparticlesDNA nanotechnologypoly(dG)-poly(dC)
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Gennady Eidelshtein Moran Fattal Gavriel Avishai Benjamin Kempinski Clelia Giannini Alexander Kotlyar
spellingShingle	Gennady Eidelshtein Moran Fattal Gavriel Avishai Benjamin Kempinski Clelia Giannini Alexander Kotlyar Preparation, Characterization and Manipulation of Conjugates between Gold Nanoparticles and DNA Nanomaterials atomic force microscopy gold nanoparticles DNA nanotechnology poly(dG)-poly(dC)
author_facet	Gennady Eidelshtein Moran Fattal Gavriel Avishai Benjamin Kempinski Clelia Giannini Alexander Kotlyar
author_sort	Gennady Eidelshtein
title	Preparation, Characterization and Manipulation of Conjugates between Gold Nanoparticles and DNA
title_short	Preparation, Characterization and Manipulation of Conjugates between Gold Nanoparticles and DNA
title_full	Preparation, Characterization and Manipulation of Conjugates between Gold Nanoparticles and DNA
title_fullStr	Preparation, Characterization and Manipulation of Conjugates between Gold Nanoparticles and DNA
title_full_unstemmed	Preparation, Characterization and Manipulation of Conjugates between Gold Nanoparticles and DNA
title_sort	preparation, characterization and manipulation of conjugates between gold nanoparticles and dna
publisher	MDPI AG
series	Nanomaterials
issn	2079-4991
publishDate	2016-09-01
description	Here we described the preparation and characterization by atomic force microscopy of dumbbell-shaped conjugates between 450 bp double-stranded DNA polymer, poly(dG)-poly(dC), and 5 nm gold nanoparticles (GNPs). We have demonstrated that the size of the nanoparticles in the conjugates can be increased in a controlled fashion. Application of the conjugates for measuring the electrical conductivity of DNA is discussed.
topic	atomic force microscopy gold nanoparticles DNA nanotechnology poly(dG)-poly(dC)
url	http://www.mdpi.com/2079-4991/6/9/167
work_keys_str_mv	AT gennadyeidelshtein preparationcharacterizationandmanipulationofconjugatesbetweengoldnanoparticlesanddna AT moranfattal preparationcharacterizationandmanipulationofconjugatesbetweengoldnanoparticlesanddna AT gavrielavishai preparationcharacterizationandmanipulationofconjugatesbetweengoldnanoparticlesanddna AT benjaminkempinski preparationcharacterizationandmanipulationofconjugatesbetweengoldnanoparticlesanddna AT cleliagiannini preparationcharacterizationandmanipulationofconjugatesbetweengoldnanoparticlesanddna AT alexanderkotlyar preparationcharacterizationandmanipulationofconjugatesbetweengoldnanoparticlesanddna
_version_	1725678709869903872

Preparation, Characterization and Manipulation of Conjugates between Gold Nanoparticles and DNA

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