Characteristics of the ATP-Induced Ca2+-Entry Pathway in the t-BBEC 117 Cell Line Derived From Bovine Brain Endothelial Cells

Characteristics of the ATP-Induced Ca2+-Entry Pathway in the t-BBEC 117 Cell Line Derived From Bovine Brain Endothelial Cells

ATP-receptor (P2Y) stimulation induced sustained Ca2+-entry, which was essential for the enhanced cell-proliferation in t-BBEC117, an immortalized cell-line derived from bovine brain endothelial cells. Application of Ca2+ following store-depletion with thapsigargin in Ca2+-free solution induced Ca2+...

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Main Authors: Daiju Yamazaki, Susumu Ohya, Kiyofumi Asai, Yuji Imaizumi
Format: Article
Language:English
Published: Elsevier 2007-01-01
Series:Journal of Pharmacological Sciences
Online Access:http://www.sciencedirect.com/science/article/pii/S1347861319342859
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spelling doaj-a678c5e2b61946cbbcddacbbe2ec096c2020-11-25T01:52:44ZengElsevierJournal of Pharmacological Sciences1347-86132007-01-011041103107Characteristics of the ATP-Induced Ca2+-Entry Pathway in the t-BBEC 117 Cell Line Derived From Bovine Brain Endothelial CellsDaiju Yamazaki0Susumu Ohya1Kiyofumi Asai2Yuji Imaizumi3Department of Molecular & Cellular Pharmacology, Graduate School of Pharmaceutical Sciences, Nagoya City University, 3-1 Tanabedori, Mizuho-ku, Nagoya 467-8603, JapanDepartment of Molecular & Cellular Pharmacology, Graduate School of Pharmaceutical Sciences, Nagoya City University, 3-1 Tanabedori, Mizuho-ku, Nagoya 467-8603, JapanDepartment of Molecular Neurobiology, Graduate School of Medical Sciences, Nagoya City University, 1 Kawasumi, Mizuho-cho, Mizuho-ku, Nagoya 467-8601, JapanDepartment of Molecular & Cellular Pharmacology, Graduate School of Pharmaceutical Sciences, Nagoya City University, 3-1 Tanabedori, Mizuho-ku, Nagoya 467-8603, Japan; Corresponding author. yimaizum@phar.nagoya-cu.ac.jpATP-receptor (P2Y) stimulation induced sustained Ca2+-entry, which was essential for the enhanced cell-proliferation in t-BBEC117, an immortalized cell-line derived from bovine brain endothelial cells. Application of Ca2+ following store-depletion with thapsigargin in Ca2+-free solution induced Ca2+-entry through store-operated channels (SOCs). Ca2+-entry induced by ATP or 1-oleoyl-2-acetyl-sn-glycerol (OAG) together with Ca2+ was significantly larger than that by Ca2+ alone, suggesting the involvement of receptor-operated channels (ROCs) in the Ca2+-entry. Results obtained using pharmacological tools suggest that the contribution of Ca2+ sources to ATP-induced [Ca2+]i rise in t-BBEC117 is estimated as approximately 2:1:2 for Ca2+-release and Ca2+-entry though SOCs and ROCs, respectively. Keywords:: brain capillary endothelial cell, store-operated channel, receptor-operated channelhttp://www.sciencedirect.com/science/article/pii/S1347861319342859
collection DOAJ
language English
format Article
sources DOAJ
author Daiju Yamazaki
Susumu Ohya
Kiyofumi Asai
Yuji Imaizumi
spellingShingle Daiju Yamazaki
Susumu Ohya
Kiyofumi Asai
Yuji Imaizumi
Characteristics of the ATP-Induced Ca2+-Entry Pathway in the t-BBEC 117 Cell Line Derived From Bovine Brain Endothelial Cells
Journal of Pharmacological Sciences
author_facet Daiju Yamazaki
Susumu Ohya
Kiyofumi Asai
Yuji Imaizumi
author_sort Daiju Yamazaki
title Characteristics of the ATP-Induced Ca2+-Entry Pathway in the t-BBEC 117 Cell Line Derived From Bovine Brain Endothelial Cells
title_short Characteristics of the ATP-Induced Ca2+-Entry Pathway in the t-BBEC 117 Cell Line Derived From Bovine Brain Endothelial Cells
title_full Characteristics of the ATP-Induced Ca2+-Entry Pathway in the t-BBEC 117 Cell Line Derived From Bovine Brain Endothelial Cells
title_fullStr Characteristics of the ATP-Induced Ca2+-Entry Pathway in the t-BBEC 117 Cell Line Derived From Bovine Brain Endothelial Cells
title_full_unstemmed Characteristics of the ATP-Induced Ca2+-Entry Pathway in the t-BBEC 117 Cell Line Derived From Bovine Brain Endothelial Cells
title_sort characteristics of the atp-induced ca2+-entry pathway in the t-bbec 117 cell line derived from bovine brain endothelial cells
publisher Elsevier
series Journal of Pharmacological Sciences
issn 1347-8613
publishDate 2007-01-01
description ATP-receptor (P2Y) stimulation induced sustained Ca2+-entry, which was essential for the enhanced cell-proliferation in t-BBEC117, an immortalized cell-line derived from bovine brain endothelial cells. Application of Ca2+ following store-depletion with thapsigargin in Ca2+-free solution induced Ca2+-entry through store-operated channels (SOCs). Ca2+-entry induced by ATP or 1-oleoyl-2-acetyl-sn-glycerol (OAG) together with Ca2+ was significantly larger than that by Ca2+ alone, suggesting the involvement of receptor-operated channels (ROCs) in the Ca2+-entry. Results obtained using pharmacological tools suggest that the contribution of Ca2+ sources to ATP-induced [Ca2+]i rise in t-BBEC117 is estimated as approximately 2:1:2 for Ca2+-release and Ca2+-entry though SOCs and ROCs, respectively. Keywords:: brain capillary endothelial cell, store-operated channel, receptor-operated channel
url http://www.sciencedirect.com/science/article/pii/S1347861319342859
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AT kiyofumiasai characteristicsoftheatpinducedca2entrypathwayinthetbbec117celllinederivedfrombovinebrainendothelialcells
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