Antibacterial Activity of Indian Borage (Plectranthus amboinicus Benth) Leaf Extracts in Food Systems and Against Natural Microflora in Chicken Meat
The ability of acetone and ethyl acetate extracts of the leaves of a traditional Indian medicinal plant, Indian borage (Plectranthus amboinicus Benth) to prevent spoilage of artificially inoculated model food systems (cabbage and papaya) and natural microflora of chicken meat was evaluated. These ex...
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University of Zagreb
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doaj-a668c9eacffd43158ec6ceb5639b1b8a2020-11-25T03:20:18ZengUniversity of ZagrebFood Technology and Biotechnology1330-98621334-26062016-01-015419096Antibacterial Activity of Indian Borage (Plectranthus amboinicus Benth) Leaf Extracts in Food Systems and Against Natural Microflora in Chicken MeatSandeep Kumar Gupta0Pradeep Singh Negi1Fruit and Vegetable Technology Department, CSIR-Central Food Technological Research Institute, Mysore, IndiaFruit and Vegetable Technology Department, CSIR-Central Food Technological Research Institute, Mysore, IndiaThe ability of acetone and ethyl acetate extracts of the leaves of a traditional Indian medicinal plant, Indian borage (Plectranthus amboinicus Benth) to prevent spoilage of artificially inoculated model food systems (cabbage and papaya) and natural microflora of chicken meat was evaluated. These extracts were able to reduce the bacterial counts in all food systems; however, the effective concentration varied with the complexity of the system (in descending order: cabbage, papaya and chicken). A probable mode of action of extracts was investigated by analyzing the changes they cause in bacterial cell wall and leakage of nucleic acid from bacterial cells. Both acetone and ethyl acetate extracts at their respective minimum inhibitory concentrations resulted in leakage of cell constituents to an extent of 40 to 80 and 60 to 95 %, respectively, compared to the control, and finally leading to disintegration of cell walls. These findings indicate the potential use of ethyl acetate and acetone extracts of Indian borage leaves in food preservation.http://hrcak.srce.hr/file/228627antibacterial activitycell wall damagefood model systemfood safetyPlectranthus amboinicus |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Sandeep Kumar Gupta Pradeep Singh Negi |
spellingShingle |
Sandeep Kumar Gupta Pradeep Singh Negi Antibacterial Activity of Indian Borage (Plectranthus amboinicus Benth) Leaf Extracts in Food Systems and Against Natural Microflora in Chicken Meat Food Technology and Biotechnology antibacterial activity cell wall damage food model system food safety Plectranthus amboinicus |
author_facet |
Sandeep Kumar Gupta Pradeep Singh Negi |
author_sort |
Sandeep Kumar Gupta |
title |
Antibacterial Activity of Indian Borage (Plectranthus amboinicus Benth) Leaf Extracts in Food Systems and Against Natural Microflora in Chicken Meat |
title_short |
Antibacterial Activity of Indian Borage (Plectranthus amboinicus Benth) Leaf Extracts in Food Systems and Against Natural Microflora in Chicken Meat |
title_full |
Antibacterial Activity of Indian Borage (Plectranthus amboinicus Benth) Leaf Extracts in Food Systems and Against Natural Microflora in Chicken Meat |
title_fullStr |
Antibacterial Activity of Indian Borage (Plectranthus amboinicus Benth) Leaf Extracts in Food Systems and Against Natural Microflora in Chicken Meat |
title_full_unstemmed |
Antibacterial Activity of Indian Borage (Plectranthus amboinicus Benth) Leaf Extracts in Food Systems and Against Natural Microflora in Chicken Meat |
title_sort |
antibacterial activity of indian borage (plectranthus amboinicus benth) leaf extracts in food systems and against natural microflora in chicken meat |
publisher |
University of Zagreb |
series |
Food Technology and Biotechnology |
issn |
1330-9862 1334-2606 |
publishDate |
2016-01-01 |
description |
The ability of acetone and ethyl acetate extracts of the leaves of a traditional Indian medicinal plant, Indian borage (Plectranthus amboinicus Benth) to prevent spoilage of artificially inoculated model food systems (cabbage and papaya) and natural microflora of chicken meat was evaluated. These extracts were able to reduce the bacterial counts in all food systems; however, the effective concentration varied with the complexity of the system (in descending order: cabbage, papaya and chicken). A probable mode of action of extracts was investigated by analyzing the changes they cause in bacterial cell wall and leakage of nucleic acid from bacterial cells. Both acetone and ethyl acetate extracts at their respective minimum inhibitory concentrations resulted in leakage of cell constituents to an extent of 40 to 80 and 60 to 95 %, respectively, compared to the control, and finally leading to disintegration of cell walls. These findings indicate the potential use of ethyl acetate and acetone extracts of Indian borage leaves in food preservation. |
topic |
antibacterial activity cell wall damage food model system food safety Plectranthus amboinicus |
url |
http://hrcak.srce.hr/file/228627 |
work_keys_str_mv |
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