Antibacterial Activity of Indian Borage (Plectranthus amboinicus Benth) Leaf Extracts in Food Systems and Against Natural Microflora in Chicken Meat

The ability of acetone and ethyl acetate extracts of the leaves of a traditional Indian medicinal plant, Indian borage (Plectranthus amboinicus Benth) to prevent spoilage of artificially inoculated model food systems (cabbage and papaya) and natural microflora of chicken meat was evaluated. These ex...

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Main Authors: Sandeep Kumar Gupta, Pradeep Singh Negi
Format: Article
Language:English
Published: University of Zagreb 2016-01-01
Series:Food Technology and Biotechnology
Subjects:
Online Access:http://hrcak.srce.hr/file/228627
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spelling doaj-a668c9eacffd43158ec6ceb5639b1b8a2020-11-25T03:20:18ZengUniversity of ZagrebFood Technology and Biotechnology1330-98621334-26062016-01-015419096Antibacterial Activity of Indian Borage (Plectranthus amboinicus Benth) Leaf Extracts in Food Systems and Against Natural Microflora in Chicken MeatSandeep Kumar Gupta0Pradeep Singh Negi1Fruit and Vegetable Technology Department, CSIR-Central Food Technological Research Institute, Mysore, IndiaFruit and Vegetable Technology Department, CSIR-Central Food Technological Research Institute, Mysore, IndiaThe ability of acetone and ethyl acetate extracts of the leaves of a traditional Indian medicinal plant, Indian borage (Plectranthus amboinicus Benth) to prevent spoilage of artificially inoculated model food systems (cabbage and papaya) and natural microflora of chicken meat was evaluated. These extracts were able to reduce the bacterial counts in all food systems; however, the effective concentration varied with the complexity of the system (in descending order: cabbage, papaya and chicken). A probable mode of action of extracts was investigated by analyzing the changes they cause in bacterial cell wall and leakage of nucleic acid from bacterial cells. Both acetone and ethyl acetate extracts at their respective minimum inhibitory concentrations resulted in leakage of cell constituents to an extent of 40 to 80 and 60 to 95 %, respectively, compared to the control, and finally leading to disintegration of cell walls. These findings indicate the potential use of ethyl acetate and acetone extracts of Indian borage leaves in food preservation.http://hrcak.srce.hr/file/228627antibacterial activitycell wall damagefood model systemfood safetyPlectranthus amboinicus
collection DOAJ
language English
format Article
sources DOAJ
author Sandeep Kumar Gupta
Pradeep Singh Negi
spellingShingle Sandeep Kumar Gupta
Pradeep Singh Negi
Antibacterial Activity of Indian Borage (Plectranthus amboinicus Benth) Leaf Extracts in Food Systems and Against Natural Microflora in Chicken Meat
Food Technology and Biotechnology
antibacterial activity
cell wall damage
food model system
food safety
Plectranthus amboinicus
author_facet Sandeep Kumar Gupta
Pradeep Singh Negi
author_sort Sandeep Kumar Gupta
title Antibacterial Activity of Indian Borage (Plectranthus amboinicus Benth) Leaf Extracts in Food Systems and Against Natural Microflora in Chicken Meat
title_short Antibacterial Activity of Indian Borage (Plectranthus amboinicus Benth) Leaf Extracts in Food Systems and Against Natural Microflora in Chicken Meat
title_full Antibacterial Activity of Indian Borage (Plectranthus amboinicus Benth) Leaf Extracts in Food Systems and Against Natural Microflora in Chicken Meat
title_fullStr Antibacterial Activity of Indian Borage (Plectranthus amboinicus Benth) Leaf Extracts in Food Systems and Against Natural Microflora in Chicken Meat
title_full_unstemmed Antibacterial Activity of Indian Borage (Plectranthus amboinicus Benth) Leaf Extracts in Food Systems and Against Natural Microflora in Chicken Meat
title_sort antibacterial activity of indian borage (plectranthus amboinicus benth) leaf extracts in food systems and against natural microflora in chicken meat
publisher University of Zagreb
series Food Technology and Biotechnology
issn 1330-9862
1334-2606
publishDate 2016-01-01
description The ability of acetone and ethyl acetate extracts of the leaves of a traditional Indian medicinal plant, Indian borage (Plectranthus amboinicus Benth) to prevent spoilage of artificially inoculated model food systems (cabbage and papaya) and natural microflora of chicken meat was evaluated. These extracts were able to reduce the bacterial counts in all food systems; however, the effective concentration varied with the complexity of the system (in descending order: cabbage, papaya and chicken). A probable mode of action of extracts was investigated by analyzing the changes they cause in bacterial cell wall and leakage of nucleic acid from bacterial cells. Both acetone and ethyl acetate extracts at their respective minimum inhibitory concentrations resulted in leakage of cell constituents to an extent of 40 to 80 and 60 to 95 %, respectively, compared to the control, and finally leading to disintegration of cell walls. These findings indicate the potential use of ethyl acetate and acetone extracts of Indian borage leaves in food preservation.
topic antibacterial activity
cell wall damage
food model system
food safety
Plectranthus amboinicus
url http://hrcak.srce.hr/file/228627
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AT pradeepsinghnegi antibacterialactivityofindianborageplectranthusamboinicusbenthleafextractsinfoodsystemsandagainstnaturalmicroflorainchickenmeat
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