Dissecting myosin-5B mechanosensitivity and calcium regulation at the single molecule level

Dissecting myosin-5B mechanosensitivity and calcium regulation at the single molecule level

Myosin-5B is an actin-based motor important for endosome recycling, but the molecular mechanism underlying its motility remains unknown. Here authors use single molecule imaging and high-speed laser tweezers to dissect the mechanoenzymatic properties of myosin-5B, which shows processive motility wit...

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Main Authors: Lucia Gardini, Sarah M. Heissler, Claudia Arbore, Yi Yang, James R. Sellers, Francesco S. Pavone, Marco Capitanio
Format: Article
Language:English
Published: Nature Publishing Group 2018-07-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-018-05251-z
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spelling doaj-a663d8e49b69440c972ca488748037c22021-05-11T10:29:42ZengNature Publishing GroupNature Communications2041-17232018-07-019111210.1038/s41467-018-05251-zDissecting myosin-5B mechanosensitivity and calcium regulation at the single molecule levelLucia Gardini0Sarah M. Heissler1Claudia Arbore2Yi Yang3James R. Sellers4Francesco S. Pavone5Marco Capitanio6LENS—European Laboratory for Non-linear Spectroscopy, University of FlorenceLaboratory of Molecular Physiology, National Heart, Lung, and Blood Institute, National Institutes of HealthLENS—European Laboratory for Non-linear Spectroscopy, University of FlorenceLaboratory of Molecular Physiology, National Heart, Lung, and Blood Institute, National Institutes of HealthLaboratory of Molecular Physiology, National Heart, Lung, and Blood Institute, National Institutes of HealthLENS—European Laboratory for Non-linear Spectroscopy, University of FlorenceLENS—European Laboratory for Non-linear Spectroscopy, University of FlorenceMyosin-5B is an actin-based motor important for endosome recycling, but the molecular mechanism underlying its motility remains unknown. Here authors use single molecule imaging and high-speed laser tweezers to dissect the mechanoenzymatic properties of myosin-5B, which shows processive motility with peculiar mechanosensitivity.https://doi.org/10.1038/s41467-018-05251-z
collection DOAJ
language English
format Article
sources DOAJ
author Lucia Gardini
Sarah M. Heissler
Claudia Arbore
Yi Yang
James R. Sellers
Francesco S. Pavone
Marco Capitanio
spellingShingle Lucia Gardini
Sarah M. Heissler
Claudia Arbore
Yi Yang
James R. Sellers
Francesco S. Pavone
Marco Capitanio
Dissecting myosin-5B mechanosensitivity and calcium regulation at the single molecule level
Nature Communications
author_facet Lucia Gardini
Sarah M. Heissler
Claudia Arbore
Yi Yang
James R. Sellers
Francesco S. Pavone
Marco Capitanio
author_sort Lucia Gardini
title Dissecting myosin-5B mechanosensitivity and calcium regulation at the single molecule level
title_short Dissecting myosin-5B mechanosensitivity and calcium regulation at the single molecule level
title_full Dissecting myosin-5B mechanosensitivity and calcium regulation at the single molecule level
title_fullStr Dissecting myosin-5B mechanosensitivity and calcium regulation at the single molecule level
title_full_unstemmed Dissecting myosin-5B mechanosensitivity and calcium regulation at the single molecule level
title_sort dissecting myosin-5b mechanosensitivity and calcium regulation at the single molecule level
publisher Nature Publishing Group
series Nature Communications
issn 2041-1723
publishDate 2018-07-01
description Myosin-5B is an actin-based motor important for endosome recycling, but the molecular mechanism underlying its motility remains unknown. Here authors use single molecule imaging and high-speed laser tweezers to dissect the mechanoenzymatic properties of myosin-5B, which shows processive motility with peculiar mechanosensitivity.
url https://doi.org/10.1038/s41467-018-05251-z
work_keys_str_mv AT luciagardini dissectingmyosin5bmechanosensitivityandcalciumregulationatthesinglemoleculelevel
AT sarahmheissler dissectingmyosin5bmechanosensitivityandcalciumregulationatthesinglemoleculelevel
AT claudiaarbore dissectingmyosin5bmechanosensitivityandcalciumregulationatthesinglemoleculelevel
AT yiyang dissectingmyosin5bmechanosensitivityandcalciumregulationatthesinglemoleculelevel
AT jamesrsellers dissectingmyosin5bmechanosensitivityandcalciumregulationatthesinglemoleculelevel
AT francescospavone dissectingmyosin5bmechanosensitivityandcalciumregulationatthesinglemoleculelevel
AT marcocapitanio dissectingmyosin5bmechanosensitivityandcalciumregulationatthesinglemoleculelevel
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