Inflammatory response of the uterus after insemination in cows of the Ecuadorian Amazon

• Main Authors: R. Quinteros-Pozo, D. Yánez-Avalos, J. López-Parra, M. Ortega, J. Masaquiza, S. Bernardi, Pablo Marini
• Format: Article
• Language: Spanish
• Published: Asociación Peruana de Reproducción Animal 2019-12-01
• Series: SPERMOVA
• Subjects: neutrophils; spermatozoa; cows; uterus
• Online Access: http://spermova.pe/site2/files/Revistas/Rev.%209%20Vol.%202/3_Quinteros-pozo_2019_in_press.pdf
• ISSN: 2223-9375; 2308-4928

The objective of this study was to evaluate the response of the polymorphonuclear neutrophils after an artificial insemination with different components in cows of the Ecuadorian Amazon. On October 20, 2018, 12 crossed dairy cows were used which belong to the dairy rodeo of the Centre of Research, Postgraduate and Conservation of the Amazon Biodiversity. The cows were all randomly divided in a similar period into three groups: cows, inseminated with distilled water (n = 4); cows, inseminated with diluent by Triladyl © (n = 4) and cows, inseminated with conventional semen, tested and with high fertilization marketed by Showcase Selections (n = 4). Before proceeding with the artificial insemination, cytological samples were taken from every cow using the technology of cytobrush. After the insemination of the three groups, the cytobrush and blood samples were repeated at 4 hours, 8 hours and 12 hours after insemination, having a total of 48 repetitions. The response of the N-PMN is observed for each treatment, showing that there are only significant differences (P≤0.05) in which semen is used in an artificial insemination. Showing a peak of N-PMN at 8 hours after being inseminated, it is exceeding the 5% limit to consider it a subclinical endometritis. It is concluded that there is a response of polymorphonuclear neutrophils after the artificial insemination with semen in cows of the Ecuadorian Amazon.