Expressed repeat elements improve RT-qPCR normalization across a wide range of zebrafish gene expression studies.

The selection and validation of stably expressed reference genes is a critical issue for proper RT-qPCR data normalization. In zebrafish expression studies, many commonly used reference genes are not generally applicable given their variability in expression levels under a variety of experimental co...

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Bibliographic Details
Main Authors: Suzanne Vanhauwaert, Gert Van Peer, Ali Rihani, Els Janssens, Pieter Rondou, Steve Lefever, Anne De Paepe, Paul J Coucke, Frank Speleman, Jo Vandesompele, Andy Willaert
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2014-01-01
Series:PLoS ONE
Online Access:https://doi.org/10.1371/journal.pone.0109091