Regulation of microRNA-33, SREBP and ABCA1 genes in a mouse model of high cholesterol
<p>MicroRNAs are short non-coding RNAs that regulate gene expression. Several microRNAs, useful for coronary artery disease assessment, have previously been identified. MicroRNA-33 is located within SREBP introns and controls cholesterol homeostasis. In order to find the possibility of microR...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Copernicus Publications
2021-03-01
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| Series: | Archives Animal Breeding |
| Online Access: | https://aab.copernicus.org/articles/64/103/2021/aab-64-103-2021.pdf |
| Summary: | <p>MicroRNAs are short non-coding RNAs that regulate gene expression.
Several microRNAs, useful for coronary artery disease assessment, have
previously been identified. MicroRNA-33 is located within SREBP introns and
controls cholesterol homeostasis. In order to find the possibility of
microRNA-33 as a potential biomarker in high cholesterol disease, we
developed a mouse model for coronary heart disease by feeding mice with a
high-fat diet. The expression differences of microRNA-33, SREBP and ABCA1
genes in the liver, muscle, and lipid tissues were compared between a
high-cholesterol group and control group in mice. The results showed that
ABCA1 was up-regulated by high cholesterol conditions in liver, muscle and
lipid tissues. SREBP1C was up-regulated by high cholesterol conditions in
the liver and lipid tissues and down-regulated by high cholesterol
conditions in the muscle tissue. MicroRNA-33 and SREBP2 were down-regulated
by high cholesterol conditions in the liver and muscle tissues and
up-regulated by high cholesterol conditions in the lipid tissue. Our study
suggests that antisense therapeutic targeting of microRNA-33 may be a
potential biomarker for cardiovascular disease.</p> |
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| ISSN: | 0003-9438 2363-9822 |