Evaluation of Relative Frequency of Single Nucleotide Polymorphism G870A (rs9344) in CyclinD1 Gene in Colorectal Cancer Patients and Healthy Subjects of Isfahan Province, Iran

• Main Authors: Shima Rahimirad, Rasoul Salehi, Behnaz Saffar, Mostafa Niaee
• Format: Article
• Language: fas
• Published: Vesnu Publications 2016-08-01
• Series: مجله دانشکده پزشکی اصفهان
• Subjects: Colorectal cancer; Single nucleotide polymorphism; CyclinD1 gene
• Online Access: http://jims.mui.ac.ir/index.php/jims/article/view/5995
• ISSN: 1027-7595; 1735-854X

Background: Colorectal cancer (CRC) is one of the most common cancers in the world. High activity of CyclinD1 gene has been seen in the progress of this cancer. A common polymorphism (G870A) in exon 4 of CyclinD1 produces a variant transcript with longer half-life and may cause uncontrollable cellular growth thus contributing to cancer development. This study was performed to evaluate the frequency of CCND1 G870A polymorphism between CRC cases and controls. Methods: DNA samples from peripheral blood leukocytes of 50 patients with sporadic colorectal cancer and 50 healthy subjects were extracted. CCND1 G870A polymorphism was genotyped by polymerase chain reaction -restriction fragment length polymorphism (PCR-RFLP) in healthy subjects and patients. Statistical analysis was performed by chi-squared test via SPSS software. Findings: Relationship between CCND1 G870A polymorphism in allele frequencies between cases and controls were not observed (P = 0.204). While the frequency of AA genotype was significantly higher in patients (P = 0.040, 95% CI = 1.13-5.54, OR = 2.25). Conclusion: According to the significant association observed between certain genotypes of this locus (AA) with colorectal cancer, it can be used in the future as a good prognostic marker in CRC screening programs.