Purification and Characterization of Streptomyces sp. IK Chitinase

Purification and Characterization of Streptomyces sp. IK Chitinase

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Streptomyces sp. IK isolated from compost inoculants, could produce extra cellular chitinase in a medium containing 0.2% (w/v) colloidal chitin, fermented for 96 hours at 30oC. The enzyme was purified by a combination of ammonium sulphate precipitation and DEAE-Cellulose anion-exchange chromatograph...

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Main Authors: Sebastian Margino, Agustinus Joko Nugroho, Widya Asmara
Format: Article
Language:English
Published: Universitas Gadjah Mada, Yogyakarta 2015-11-01
Series:Indonesian Journal of Biotechnology
Online Access:http://journal.ugm.ac.id/ijbiotech/article/view/7820
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spelling doaj-a5ed2d48d31c42cab1d1c3ea68eeede32020-11-25T00:54:37ZengUniversitas Gadjah Mada, YogyakartaIndonesian Journal of Biotechnology0853-86542089-22412015-11-011516536Purification and Characterization of Streptomyces sp. IK ChitinaseSebastian MarginoAgustinus Joko NugrohoWidya AsmaraStreptomyces sp. IK isolated from compost inoculants, could produce extra cellular chitinase in a medium containing 0.2% (w/v) colloidal chitin, fermented for 96 hours at 30oC. The enzyme was purified by a combination of ammonium sulphate precipitation and DEAE-Cellulose anion-exchange chromatography. On SDS-polyacrylamide gel electrophoresis analysis, the purified enzyme showed a mass of 71 kDa. Chitinase was optimally active at pH of 6.7 and at 37oC. Km value and Vmax of the protein for colloidal chitin were 2.92 mg/ml and 4.26 &igrave;g/h, respectively.<br /><br />Key words : chitinase, Streptomyces, purification, characterizationhttp://journal.ugm.ac.id/ijbiotech/article/view/7820
collection DOAJ
language English
format Article
sources DOAJ
author Sebastian Margino
Agustinus Joko Nugroho
Widya Asmara
spellingShingle Sebastian Margino
Agustinus Joko Nugroho
Widya Asmara
Purification and Characterization of Streptomyces sp. IK Chitinase
Indonesian Journal of Biotechnology
author_facet Sebastian Margino
Agustinus Joko Nugroho
Widya Asmara
author_sort Sebastian Margino
title Purification and Characterization of Streptomyces sp. IK Chitinase
title_short Purification and Characterization of Streptomyces sp. IK Chitinase
title_full Purification and Characterization of Streptomyces sp. IK Chitinase
title_fullStr Purification and Characterization of Streptomyces sp. IK Chitinase
title_full_unstemmed Purification and Characterization of Streptomyces sp. IK Chitinase
title_sort purification and characterization of streptomyces sp. ik chitinase
publisher Universitas Gadjah Mada, Yogyakarta
series Indonesian Journal of Biotechnology
issn 0853-8654
2089-2241
publishDate 2015-11-01
description Streptomyces sp. IK isolated from compost inoculants, could produce extra cellular chitinase in a medium containing 0.2% (w/v) colloidal chitin, fermented for 96 hours at 30oC. The enzyme was purified by a combination of ammonium sulphate precipitation and DEAE-Cellulose anion-exchange chromatography. On SDS-polyacrylamide gel electrophoresis analysis, the purified enzyme showed a mass of 71 kDa. Chitinase was optimally active at pH of 6.7 and at 37oC. Km value and Vmax of the protein for colloidal chitin were 2.92 mg/ml and 4.26 &igrave;g/h, respectively.<br /><br />Key words : chitinase, Streptomyces, purification, characterization
url http://journal.ugm.ac.id/ijbiotech/article/view/7820
work_keys_str_mv AT sebastianmargino purificationandcharacterizationofstreptomycesspikchitinase
AT agustinusjokonugroho purificationandcharacterizationofstreptomycesspikchitinase
AT widyaasmara purificationandcharacterizationofstreptomycesspikchitinase
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