Green synthesis of silver nanoparticles using methanolic fruit extract of Aegle marmelos and their antimicrobial potential against human bacterial pathogens

Green synthesis of silver nanoparticles using methanolic fruit extract of Aegle marmelos and their antimicrobial potential against human bacterial pathogens

Plant-based synthesis of nanoparticles has generated worldwide interest because of cost-effectiveness, eco-friendly nature and plethora of applications. In the present investigation, antimicrobial potential of silver nanoparticles (AgNPs) of methanolic extract of Aegle marmelos fruit has been invest...

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Main Authors: Mamta Devi, Shikha Devi, Vaishali Sharma, Nidhi Rana, Ravi Kant Bhatia, Arvind Kumar Bhatt
Format: Article
Language:English
Published: Elsevier 2020-03-01
Series:Journal of Traditional and Complementary Medicine
Online Access:http://www.sciencedirect.com/science/article/pii/S2225411018307004
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spelling doaj-a5aa7cb5480f461eab4ee5f6240b8fe92020-11-25T02:04:21ZengElsevierJournal of Traditional and Complementary Medicine2225-41102020-03-01102158165Green synthesis of silver nanoparticles using methanolic fruit extract of Aegle marmelos and their antimicrobial potential against human bacterial pathogensMamta Devi0Shikha Devi1Vaishali Sharma2Nidhi Rana3Ravi Kant Bhatia4Arvind Kumar Bhatt5Department of Biotechnology, Himachal Pradesh University, Summerhill, Shimla, 171005, IndiaDepartment of Biotechnology, Himachal Pradesh University, Summerhill, Shimla, 171005, IndiaDepartment of Biotechnology, Himachal Pradesh University, Summerhill, Shimla, 171005, IndiaDepartment of Biotechnology, Himachal Pradesh University, Summerhill, Shimla, 171005, IndiaDepartment of Biotechnology, Himachal Pradesh University, Summerhill, Shimla, 171005, IndiaCorresponding author.; Department of Biotechnology, Himachal Pradesh University, Summerhill, Shimla, 171005, IndiaPlant-based synthesis of nanoparticles has generated worldwide interest because of cost-effectiveness, eco-friendly nature and plethora of applications. In the present investigation, antimicrobial potential of silver nanoparticles (AgNPs) of methanolic extract of Aegle marmelos fruit has been investigated. Agar well diffusion method was used for determining antimicrobial activity of solvent extracts (viz., petroleum ether, chloroform, acetone, methanol and aqueous), and AgNPs. Among these, methanolic extract of A. marmelos showed highest inhibitory activity against B. cereus (16.17 ± 0.50 mm) followed by P. aeruginosa (13.33 ± 0.62 mm) and E. coli. Phytochemical analysis of methanolic extract of A. marmelos revealed the presence of tannins, saponins, steroids, alkaloids, flavonoids, and glycosides. AgNPs synthesized using A. marmelos methanolic extract, characterized by UV–Visible spectroscopy, atomic force microscopy, dynamic light scattering, and X-ray diffraction showed a peak at 436 nm and size ranged between 159 and 181 nm. Evaluation of the antimicrobial potential of green synthesized AgNPs recorded the highest inhibitory activity against B. cereus (19.25 ± 0.19 mm) followed by P. aeruginosa (16.50 ± 0.30 mm) and S. dysentriae. The minimum inhibitory concentration (MIC) of synthesized AgNPs was found to be in the range of 0.009875–0.0395 mg/100 μl which was quite lower than the MIC of crude extract i.e. 0.0781–0.3125 mg/100 μl. The results obtained indicated that the different crude extracts of A. marmelos plant as well as AgNPs have a strong and effective antimicrobial potential that provide a marvelous source for the development of new drug molecules of herbal origin which may be used for the welfare of humanity. Keywords: Antimicrobial potential, Crude extract, MIC, Nanoparticle, XRD, AFMhttp://www.sciencedirect.com/science/article/pii/S2225411018307004
collection DOAJ
language English
format Article
sources DOAJ
author Mamta Devi
Shikha Devi
Vaishali Sharma
Nidhi Rana
Ravi Kant Bhatia
Arvind Kumar Bhatt
spellingShingle Mamta Devi
Shikha Devi
Vaishali Sharma
Nidhi Rana
Ravi Kant Bhatia
Arvind Kumar Bhatt
Green synthesis of silver nanoparticles using methanolic fruit extract of Aegle marmelos and their antimicrobial potential against human bacterial pathogens
Journal of Traditional and Complementary Medicine
author_facet Mamta Devi
Shikha Devi
Vaishali Sharma
Nidhi Rana
Ravi Kant Bhatia
Arvind Kumar Bhatt
author_sort Mamta Devi
title Green synthesis of silver nanoparticles using methanolic fruit extract of Aegle marmelos and their antimicrobial potential against human bacterial pathogens
title_short Green synthesis of silver nanoparticles using methanolic fruit extract of Aegle marmelos and their antimicrobial potential against human bacterial pathogens
title_full Green synthesis of silver nanoparticles using methanolic fruit extract of Aegle marmelos and their antimicrobial potential against human bacterial pathogens
title_fullStr Green synthesis of silver nanoparticles using methanolic fruit extract of Aegle marmelos and their antimicrobial potential against human bacterial pathogens
title_full_unstemmed Green synthesis of silver nanoparticles using methanolic fruit extract of Aegle marmelos and their antimicrobial potential against human bacterial pathogens
title_sort green synthesis of silver nanoparticles using methanolic fruit extract of aegle marmelos and their antimicrobial potential against human bacterial pathogens
publisher Elsevier
series Journal of Traditional and Complementary Medicine
issn 2225-4110
publishDate 2020-03-01
description Plant-based synthesis of nanoparticles has generated worldwide interest because of cost-effectiveness, eco-friendly nature and plethora of applications. In the present investigation, antimicrobial potential of silver nanoparticles (AgNPs) of methanolic extract of Aegle marmelos fruit has been investigated. Agar well diffusion method was used for determining antimicrobial activity of solvent extracts (viz., petroleum ether, chloroform, acetone, methanol and aqueous), and AgNPs. Among these, methanolic extract of A. marmelos showed highest inhibitory activity against B. cereus (16.17 ± 0.50 mm) followed by P. aeruginosa (13.33 ± 0.62 mm) and E. coli. Phytochemical analysis of methanolic extract of A. marmelos revealed the presence of tannins, saponins, steroids, alkaloids, flavonoids, and glycosides. AgNPs synthesized using A. marmelos methanolic extract, characterized by UV–Visible spectroscopy, atomic force microscopy, dynamic light scattering, and X-ray diffraction showed a peak at 436 nm and size ranged between 159 and 181 nm. Evaluation of the antimicrobial potential of green synthesized AgNPs recorded the highest inhibitory activity against B. cereus (19.25 ± 0.19 mm) followed by P. aeruginosa (16.50 ± 0.30 mm) and S. dysentriae. The minimum inhibitory concentration (MIC) of synthesized AgNPs was found to be in the range of 0.009875–0.0395 mg/100 μl which was quite lower than the MIC of crude extract i.e. 0.0781–0.3125 mg/100 μl. The results obtained indicated that the different crude extracts of A. marmelos plant as well as AgNPs have a strong and effective antimicrobial potential that provide a marvelous source for the development of new drug molecules of herbal origin which may be used for the welfare of humanity. Keywords: Antimicrobial potential, Crude extract, MIC, Nanoparticle, XRD, AFM
url http://www.sciencedirect.com/science/article/pii/S2225411018307004
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