A Comparative Survey on the Expression of Retinoblastoma Protein as A Tumor Suppressor Protein in Cancerous and Non-Cancerous Colorectal Tissue Samples Using Immunohistochemistry Method

A Comparative Survey on the Expression of Retinoblastoma Protein as A Tumor Suppressor Protein in Cancerous and Non-Cancerous Colorectal Tissue Samples Using Immunohistochemistry Method

Background: Colorectal cancer is one of the most prevalent gastrointestinal malignancies. Expression of some proteins including P53, P21, E-cadherin and β-catenin is assumed to have a prognostic role for this malignancy. In the present study we evaluated the association of retinoblastoma (RB) protei...

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Main Authors: Mehdi Nikbakht Dastjerdi, Golnaz Raeesi
Format: Article
Language:fas
Published: Vesnu Publications 2012-08-01
Series:مجله دانشکده پزشکی اصفهان
Online Access:http://jims.mui.ac.ir/index.php/jims/article/view/1963
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spelling doaj-a59834621e1540e7a159dbeaef717f252020-11-25T00:41:59ZfasVesnu Publications مجله دانشکده پزشکی اصفهان1027-75951735-854X2012-08-01301941092A Comparative Survey on the Expression of Retinoblastoma Protein as A Tumor Suppressor Protein in Cancerous and Non-Cancerous Colorectal Tissue Samples Using Immunohistochemistry MethodMehdi Nikbakht Dastjerdi0Golnaz Raeesi1Associate Professor, Department of Anatomical Sciences and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranStudent of Medicine, School of Medicine And Student Research Committee, Isfahan University of Medical Sciences, Isfahan, IranBackground: Colorectal cancer is one of the most prevalent gastrointestinal malignancies. Expression of some proteins including P53, P21, E-cadherin and β-catenin is assumed to have a prognostic role for this malignancy. In the present study we evaluated the association of retinoblastoma (RB) protein expression and pathogenesis of colorectal cancer. The presence of RB in cancerous and non-cancerous colorectal tissue samples were compared using immunohistochemistry method. Methods: Tissue sections were taken from tumoral and non-tumoral defined areas of 50 colorectal cancer patients. After coloration according to immunohistochemistry method, all samples were studied for RB expression. Findings: We found RB expression in colorectal tumoral cells to be significantly higher than that of non-tumoral cells (P < 0.0001). Conclusion: RB overexpression in cancerous cells may lead to insensibility to apoptosis and consequent long life of affected cells as well as resistance to treatment. Keywords: Colorectal cancer, Immunohistochemistry, Tumor suppressor gene, Retinoblastomahttp://jims.mui.ac.ir/index.php/jims/article/view/1963
collection DOAJ
language fas
format Article
sources DOAJ
author Mehdi Nikbakht Dastjerdi
Golnaz Raeesi
spellingShingle Mehdi Nikbakht Dastjerdi
Golnaz Raeesi
A Comparative Survey on the Expression of Retinoblastoma Protein as A Tumor Suppressor Protein in Cancerous and Non-Cancerous Colorectal Tissue Samples Using Immunohistochemistry Method
مجله دانشکده پزشکی اصفهان
author_facet Mehdi Nikbakht Dastjerdi
Golnaz Raeesi
author_sort Mehdi Nikbakht Dastjerdi
title A Comparative Survey on the Expression of Retinoblastoma Protein as A Tumor Suppressor Protein in Cancerous and Non-Cancerous Colorectal Tissue Samples Using Immunohistochemistry Method
title_short A Comparative Survey on the Expression of Retinoblastoma Protein as A Tumor Suppressor Protein in Cancerous and Non-Cancerous Colorectal Tissue Samples Using Immunohistochemistry Method
title_full A Comparative Survey on the Expression of Retinoblastoma Protein as A Tumor Suppressor Protein in Cancerous and Non-Cancerous Colorectal Tissue Samples Using Immunohistochemistry Method
title_fullStr A Comparative Survey on the Expression of Retinoblastoma Protein as A Tumor Suppressor Protein in Cancerous and Non-Cancerous Colorectal Tissue Samples Using Immunohistochemistry Method
title_full_unstemmed A Comparative Survey on the Expression of Retinoblastoma Protein as A Tumor Suppressor Protein in Cancerous and Non-Cancerous Colorectal Tissue Samples Using Immunohistochemistry Method
title_sort comparative survey on the expression of retinoblastoma protein as a tumor suppressor protein in cancerous and non-cancerous colorectal tissue samples using immunohistochemistry method
publisher Vesnu Publications
series مجله دانشکده پزشکی اصفهان
issn 1027-7595
1735-854X
publishDate 2012-08-01
description Background: Colorectal cancer is one of the most prevalent gastrointestinal malignancies. Expression of some proteins including P53, P21, E-cadherin and β-catenin is assumed to have a prognostic role for this malignancy. In the present study we evaluated the association of retinoblastoma (RB) protein expression and pathogenesis of colorectal cancer. The presence of RB in cancerous and non-cancerous colorectal tissue samples were compared using immunohistochemistry method. Methods: Tissue sections were taken from tumoral and non-tumoral defined areas of 50 colorectal cancer patients. After coloration according to immunohistochemistry method, all samples were studied for RB expression. Findings: We found RB expression in colorectal tumoral cells to be significantly higher than that of non-tumoral cells (P < 0.0001). Conclusion: RB overexpression in cancerous cells may lead to insensibility to apoptosis and consequent long life of affected cells as well as resistance to treatment. Keywords: Colorectal cancer, Immunohistochemistry, Tumor suppressor gene, Retinoblastoma
url http://jims.mui.ac.ir/index.php/jims/article/view/1963
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