Morphometry and epidermal fas expression of unexposed aged versus young skin

Background: Identifying the molecular mechanisms of intrinsic aging is critical in developing modalities for reversal of cutaneous aging. Objective: The objective was to evaluate the expression of epidermal Fas, epidermal thickness, collagen, and elastic fibers degeneration in unexposed skin of aged...

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Main Authors: Nagwa H Abd El-Aal, Faten A Abd El-Wadood, Nayera H Moftah, Mohamed S El-Hakeem, Abdalla Y El-Shaal, Neven B Hassan
Format: Article
Language:English
Published: Wolters Kluwer Medknow Publications 2012-01-01
Series:Indian Journal of Dermatology
Subjects:
Online Access:http://www.e-ijd.org/article.asp?issn=0019-5154;year=2012;volume=57;issue=3;spage=181;epage=186;aulast=Abd
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spelling doaj-a593218785994085914bab13f7e5e26e2020-11-24T23:49:19ZengWolters Kluwer Medknow PublicationsIndian Journal of Dermatology0019-51541998-36112012-01-0157318118610.4103/0019-5154.96188Morphometry and epidermal fas expression of unexposed aged versus young skinNagwa H Abd El-AalFaten A Abd El-WadoodNayera H MoftahMohamed S El-HakeemAbdalla Y El-ShaalNeven B HassanBackground: Identifying the molecular mechanisms of intrinsic aging is critical in developing modalities for reversal of cutaneous aging. Objective: The objective was to evaluate the expression of epidermal Fas, epidermal thickness, collagen, and elastic fibers degeneration in unexposed skin of aged individuals compared with young ones. Materials and Methods: Skin biopsies were taken from normal skin of the back of 22 old subjects (age range: 48-75 years) and 15 young subjects (age range: 18-28 years). Skin sections were stained with hematoxylin and eosin, Masson trichrome, orcein. Epidermal thickness was measured with image analyzer and scoring was done for collagen and elastic fiber degeneration. Fas immunostaining was done. Quantitative and qualitative data were compared statistically between the old and young subjects. Results: A statistically significant decreased epidermal thickness was found in old compared with young skin (P<0.05). A statistically significant number of patients showed decreased epidermal thickness, density, and fragmentation of both collagen and elastic fibers in old compared with young skin (P<0.001). Epidermal Fas expression was detected in 19 of 22 old subjects (86.4%) compared with 2 of 15 young subjects (13.3%) (P<0.001). There was no statistically significant correlation between age of old subjects and each of epidermal thickness, collagen, and elastic fiber degeneration. Conclusion: The decreased epidermal thickness and morphological alteration of collagen and elastic fibers are not correlated with aging and Fas-mediated apoptosis could be involved in thinning of the epidermis in unexposed aged skin.http://www.e-ijd.org/article.asp?issn=0019-5154;year=2012;volume=57;issue=3;spage=181;epage=186;aulast=AbdFas agingimmunohistochemistrycollagen fibresmorphometryelastic fibres
collection DOAJ
language English
format Article
sources DOAJ
author Nagwa H Abd El-Aal
Faten A Abd El-Wadood
Nayera H Moftah
Mohamed S El-Hakeem
Abdalla Y El-Shaal
Neven B Hassan
spellingShingle Nagwa H Abd El-Aal
Faten A Abd El-Wadood
Nayera H Moftah
Mohamed S El-Hakeem
Abdalla Y El-Shaal
Neven B Hassan
Morphometry and epidermal fas expression of unexposed aged versus young skin
Indian Journal of Dermatology
Fas aging
immunohistochemistry
collagen fibres
morphometry
elastic fibres
author_facet Nagwa H Abd El-Aal
Faten A Abd El-Wadood
Nayera H Moftah
Mohamed S El-Hakeem
Abdalla Y El-Shaal
Neven B Hassan
author_sort Nagwa H Abd El-Aal
title Morphometry and epidermal fas expression of unexposed aged versus young skin
title_short Morphometry and epidermal fas expression of unexposed aged versus young skin
title_full Morphometry and epidermal fas expression of unexposed aged versus young skin
title_fullStr Morphometry and epidermal fas expression of unexposed aged versus young skin
title_full_unstemmed Morphometry and epidermal fas expression of unexposed aged versus young skin
title_sort morphometry and epidermal fas expression of unexposed aged versus young skin
publisher Wolters Kluwer Medknow Publications
series Indian Journal of Dermatology
issn 0019-5154
1998-3611
publishDate 2012-01-01
description Background: Identifying the molecular mechanisms of intrinsic aging is critical in developing modalities for reversal of cutaneous aging. Objective: The objective was to evaluate the expression of epidermal Fas, epidermal thickness, collagen, and elastic fibers degeneration in unexposed skin of aged individuals compared with young ones. Materials and Methods: Skin biopsies were taken from normal skin of the back of 22 old subjects (age range: 48-75 years) and 15 young subjects (age range: 18-28 years). Skin sections were stained with hematoxylin and eosin, Masson trichrome, orcein. Epidermal thickness was measured with image analyzer and scoring was done for collagen and elastic fiber degeneration. Fas immunostaining was done. Quantitative and qualitative data were compared statistically between the old and young subjects. Results: A statistically significant decreased epidermal thickness was found in old compared with young skin (P<0.05). A statistically significant number of patients showed decreased epidermal thickness, density, and fragmentation of both collagen and elastic fibers in old compared with young skin (P<0.001). Epidermal Fas expression was detected in 19 of 22 old subjects (86.4%) compared with 2 of 15 young subjects (13.3%) (P<0.001). There was no statistically significant correlation between age of old subjects and each of epidermal thickness, collagen, and elastic fiber degeneration. Conclusion: The decreased epidermal thickness and morphological alteration of collagen and elastic fibers are not correlated with aging and Fas-mediated apoptosis could be involved in thinning of the epidermis in unexposed aged skin.
topic Fas aging
immunohistochemistry
collagen fibres
morphometry
elastic fibres
url http://www.e-ijd.org/article.asp?issn=0019-5154;year=2012;volume=57;issue=3;spage=181;epage=186;aulast=Abd
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