The Potential Use of Electrochemotherapy in the Treatment of Uveal Melanoma: In Vitro Results in 3D Tumor Cultures and In Vivo Results in a Chick Embryo Model

The Potential Use of Electrochemotherapy in the Treatment of Uveal Melanoma: In Vitro Results in 3D Tumor Cultures and In Vivo Results in a Chick Embryo Model

Uveal melanoma (UM) is the most common primary intraocular tumor that arises from neoplastic melanocytes in the choroid, iris, and ciliary body. Electrochemotherapy (ECT) has been successfully established for the treatment of skin and soft tissue metastatic lesions, deep-seated tumors of the liver,...

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Main Authors: Miltiadis Fiorentzis, Arne Viestenz, Udo Siebolts, Berthold Seitz, Sarah E. Coupland, Joana Heinzelmann
Format: Article
Language:English
Published: MDPI AG 2019-09-01
Series:Cancers
Subjects:
electrochemotherapy
uveal melanoma
tumor spheroids
chick embryo chorioallantoic membrane
treatment
bleomycin
electroporation
in vitro
in vivo
Online Access:https://www.mdpi.com/2072-6694/11/9/1344
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spelling doaj-a58f2b7e0940485385b2cadfcec0d1ef2020-11-24T21:58:33ZengMDPI AGCancers2072-66942019-09-01119134410.3390/cancers11091344cancers11091344The Potential Use of Electrochemotherapy in the Treatment of Uveal Melanoma: In Vitro Results in 3D Tumor Cultures and In Vivo Results in a Chick Embryo ModelMiltiadis Fiorentzis0Arne Viestenz1Udo Siebolts2Berthold Seitz3Sarah E. Coupland4Joana Heinzelmann5Department of Ophthalmology, University Hospital Halle (Saale), Martin-Luther University Halle-Wittenberg, Ernst-Grube-Str. 40, 06120 Halle (Saale), GermanyDepartment of Ophthalmology, University Hospital Halle (Saale), Martin-Luther University Halle-Wittenberg, Ernst-Grube-Str. 40, 06120 Halle (Saale), GermanyDepartment of Pathology, University Hospital Halle (Saale), Martin-Luther University Halle-Wittenberg, Magdeburger Str. 14, 06112 Halle (Saale), GermanyDepartment of Ophthalmology, Saarland University Medical Center, Kirrberger Str. 100, 66421 Homburg/Saar, GermanyLiverpool Ocular Oncology Research Group, Department of Molecular and Clinical Cancer Medicine, Institute of Translational Medicine, University of Liverpool, West Derby Street, Liverpool L7 8TX, UKDepartment of Ophthalmology, University Hospital Halle (Saale), Martin-Luther University Halle-Wittenberg, Ernst-Grube-Str. 40, 06120 Halle (Saale), GermanyUveal melanoma (UM) is the most common primary intraocular tumor that arises from neoplastic melanocytes in the choroid, iris, and ciliary body. Electrochemotherapy (ECT) has been successfully established for the treatment of skin and soft tissue metastatic lesions, deep-seated tumors of the liver, bone metastases, and unresectable pancreas lesions. The aim of this study was to evaluate the effect of ECT in vitro in 3D spheroid culture systems in primary and metastatic UM cell lines. We also investigated the chick embryo chorioallantoic membrane (CAM) as an in vivo model system for the growth and treatment of UM tumors using ECT. The cytotoxic effect of ECT in 3D spheroids was analyzed seven days following treatment by assessment of the size and MTT [(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) tetrazolium reduction] assay. The cytotoxicity of ECT after intratumoral or intraarterial administration was evaluated histologically. In vitro and in vivo ECT caused a significant reduction in tumor size and viability compared to electroporation or chemotherapy in both sections of our study. The current results underline the effectiveness of ECT in the treatment of UM and prepare the way for further investigation of its potential application in UM.https://www.mdpi.com/2072-6694/11/9/1344electrochemotherapyuveal melanomatumor spheroidschick embryo chorioallantoic membranetreatmentbleomycinelectroporationin vitroin vivo
collection DOAJ
language English
format Article
sources DOAJ
author Miltiadis Fiorentzis
Arne Viestenz
Udo Siebolts
Berthold Seitz
Sarah E. Coupland
Joana Heinzelmann
spellingShingle Miltiadis Fiorentzis
Arne Viestenz
Udo Siebolts
Berthold Seitz
Sarah E. Coupland
Joana Heinzelmann
The Potential Use of Electrochemotherapy in the Treatment of Uveal Melanoma: In Vitro Results in 3D Tumor Cultures and In Vivo Results in a Chick Embryo Model
Cancers
electrochemotherapy
uveal melanoma
tumor spheroids
chick embryo chorioallantoic membrane
treatment
bleomycin
electroporation
in vitro
in vivo
author_facet Miltiadis Fiorentzis
Arne Viestenz
Udo Siebolts
Berthold Seitz
Sarah E. Coupland
Joana Heinzelmann
author_sort Miltiadis Fiorentzis
title The Potential Use of Electrochemotherapy in the Treatment of Uveal Melanoma: In Vitro Results in 3D Tumor Cultures and In Vivo Results in a Chick Embryo Model
title_short The Potential Use of Electrochemotherapy in the Treatment of Uveal Melanoma: In Vitro Results in 3D Tumor Cultures and In Vivo Results in a Chick Embryo Model
title_full The Potential Use of Electrochemotherapy in the Treatment of Uveal Melanoma: In Vitro Results in 3D Tumor Cultures and In Vivo Results in a Chick Embryo Model
title_fullStr The Potential Use of Electrochemotherapy in the Treatment of Uveal Melanoma: In Vitro Results in 3D Tumor Cultures and In Vivo Results in a Chick Embryo Model
title_full_unstemmed The Potential Use of Electrochemotherapy in the Treatment of Uveal Melanoma: In Vitro Results in 3D Tumor Cultures and In Vivo Results in a Chick Embryo Model
title_sort potential use of electrochemotherapy in the treatment of uveal melanoma: in vitro results in 3d tumor cultures and in vivo results in a chick embryo model
publisher MDPI AG
series Cancers
issn 2072-6694
publishDate 2019-09-01
description Uveal melanoma (UM) is the most common primary intraocular tumor that arises from neoplastic melanocytes in the choroid, iris, and ciliary body. Electrochemotherapy (ECT) has been successfully established for the treatment of skin and soft tissue metastatic lesions, deep-seated tumors of the liver, bone metastases, and unresectable pancreas lesions. The aim of this study was to evaluate the effect of ECT in vitro in 3D spheroid culture systems in primary and metastatic UM cell lines. We also investigated the chick embryo chorioallantoic membrane (CAM) as an in vivo model system for the growth and treatment of UM tumors using ECT. The cytotoxic effect of ECT in 3D spheroids was analyzed seven days following treatment by assessment of the size and MTT [(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) tetrazolium reduction] assay. The cytotoxicity of ECT after intratumoral or intraarterial administration was evaluated histologically. In vitro and in vivo ECT caused a significant reduction in tumor size and viability compared to electroporation or chemotherapy in both sections of our study. The current results underline the effectiveness of ECT in the treatment of UM and prepare the way for further investigation of its potential application in UM.
topic electrochemotherapy
uveal melanoma
tumor spheroids
chick embryo chorioallantoic membrane
treatment
bleomycin
electroporation
in vitro
in vivo
url https://www.mdpi.com/2072-6694/11/9/1344
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