| Summary: | An excess of Al<sup>3+</sup> will lead to biological disorders and even many diseases. Therefore, detecting the levels of Al<sup>3+</sup> in the human body has drawn great attention for health monitoring. The fluorescence method has been broadly applied because of high sensitivity, real-time detection, and intracellular imaging. In this work, a new probe with “turn-on„ fluorescence based on Schiff base derivative, 3,6-imine-triphenylamine-(9-ethyl) carbazole (ITEC), has been successfully synthesized and studied. The high selectivity and sensitivity of ITEC to Al<sup>3+</sup> were verified by fluorescence spectra and the detection limit was 2.19 nmol/L. A 1:2 stoichiometry of ITEC-Al<sup>3+</sup> was obtained by the <sup>1</sup>H NMR spectra and Job’s plot. Furthermore, ITEC was successfully applied to the detection of Al<sup>3+</sup> with different concentrations in living HeLa cells. The analog experiments about nature contamination of Al<sup>3+</sup> in cells and real samples were finished.
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