The Impact of IL-6 and IL-10 Gene Polymorphisms in Diffuse Large B-Cell Lymphoma Risk and Overall Survival in an Arab Population: A Case-Control Study

• Main Authors: Sohaib M. Al-Khatib, Nour Abdo, Laith N. AL-Eitan, Abdel-Hameed Al-Mistarehi, Deeb Jamil Zahran, Tariq Zuheir Kewan
• Format: Article
• Language: English
• Published: MDPI AG 2020-02-01
• Series: Cancers
• Subjects: diffuse large b-cell lymphoma; single nucleotide polymorphism; il-6; il-10; arab population
• Online Access: https://www.mdpi.com/2072-6694/12/2/382

B-cell lymphomas can be classified as Hodgkin and non-Hodgkin lymphomas. Diffuse large B-cell lymphoma (DLBCL) is the most common non-Hodgkin Lymphoma (NHL). The incidence of NHL is variable and affected by age, gender, racial, and geographic factors. There is strong evidence that the immune-regulatory cytokines have a major role in hematologic malignancies. In this study, we analyzed the relationship between seven single nucleotide polymorphisms (SNPs) in two selected cytokines (IL-6 rs1800795G &gt; C, rs1800796G &gt; C, rs1800797G &gt; A, IL-10 rs1800871G &gt; A, rs1800872G &gt; T, rs1800890A &gt; T, rs1800896T &gt; C) and the risk and overall survival of DLBCL patients in a Jordanian Arab population. One hundred and twenty-five DLBCL patients diagnosed at King Abdullah University Hospital (KAUH) from the period 2013&#8722;2018 and 238 matched healthy controls were included in the study. Genomic DNA was extracted from formalin-fixed paraffin-embedded tissues. Genotyping of the genetic polymorphisms was conducted using a sequencing protocol. Our study showed no significant differences in the distribution of all studied polymorphisms of DLBCL between patients and controls. The IL-6 rs1800797 was the only SNP to show significant survival results, DLBCL subjects with the codominant model (GG/AG/AA) genotypes and recessive model (AA genotype in comparison with the combined GG/GA genotype) had worse overall survival (<i>p</i> = 0.028 and 0.016, respectively).