Interactions of smoking with rs833061 polymorphism on the risk of non-alcoholic fat liver disease in Hubei Han population: a preliminary case–control study
Objective(s):Vascular endothelial growth factor (VEGF) has biological actions on energy homeostasis, inflammation and insulin resistance. The present study aimed to investigate the association between VEGF -460 T/C (rs833061), and +936 C/T (rs3025039) polymorphism and risk of non-alcohol fatty liver...
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doaj-a51f54d1ef714f7896d7c3c2728b103b2020-11-24T21:01:27ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences 2008-38662008-38742015-11-011811111211176050Interactions of smoking with rs833061 polymorphism on the risk of non-alcoholic fat liver disease in Hubei Han population: a preliminary case–control studyPengbo Wu0Yonglong Hua1Shiyun Tan2Ming Li3Shu Yongxiang4Guo Fang5Department of Gastroenterology, Renmin Hospital of Wuhan University, Jiefang Road, Wuhan, ChinaDepartment of Gastroenterology, Renmin Hospital of Tongcheng, Minzhu Road 120, Hubei, ChinaDepartment of Gastroenterology, Renmin Hospital of Wuhan University, Jiefang Road, Wuhan, ChinaDepartment of Gastroenterology, Renmin Hospital of Wuhan University, Jiefang Road, Wuhan, ChinaDepartment of Gastroenterology, Renmin Hospital of Wuhan University, Jiefang Road, Wuhan, ChinaDepartment of Gastroenterology, Renmin Hospital of Wuhan University, Jiefang Road, Wuhan, ChinaObjective(s):Vascular endothelial growth factor (VEGF) has biological actions on energy homeostasis, inflammation and insulin resistance. The present study aimed to investigate the association between VEGF -460 T/C (rs833061), and +936 C/T (rs3025039) polymorphism and risk of non-alcohol fatty liver disease (NAFLD) in Hubei Han population and to further explore the interactions of smoking with rs833061 and rs3025039. Materials and Methods:341 healthy controls and 246 cases were recruited. Two variants, rs833061 and rs3025039, were genotyped by polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP). The unconditional logistic regression (ULR) was performed to assess the association of the two variants with risk of NAFLD. Gene-environment interactions on the risk of NAFLD were preliminarily explored by generalized multifactor dimensionality reduction (GMDR) and further confirmed by ULR methods. Results:After adjusting for covariates, increased risk of NAFLD was observed in patients carrying CT/CC genotypes in rs833061 and rs3025039 (ORa=1.80, 95% confidence interval (CI): 1.51, 2.36, Pa=0.000; ORa=1.89, 95% CI: 1.41, 2.82, Pa=0.000, respectively). Interaction of smoking with rs833061 was found by GMDR, with maximum prediction accuracy (67.91%) and a maximum cross-validation consistency (10/10). ULR method confirmed that, smoking-positive patients with genotype CT/CC had 4.93 times risk of NAFLD compared to smoking-negative participants with genotype TT (ORadda=4.93, 95% CI:2.91, 8.54, Padda=0.000), which further confirmed synergistic effects. Conclusion: The results indicated that both rs833061 and rs3025039 are associated with NAFLD risk. Furthermore, rs833061 is likely to have an interaction with smoking, and they have synergistic effects on risk of NAFLD in Hubei Han population.http://ijbms.mums.ac.ir/pdf_6050_c476d3dbd222b2a88d8864d537ea34da.htmlInteractionsNAFLDPolymorphismsSmokingVEGF |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Pengbo Wu Yonglong Hua Shiyun Tan Ming Li Shu Yongxiang Guo Fang |
spellingShingle |
Pengbo Wu Yonglong Hua Shiyun Tan Ming Li Shu Yongxiang Guo Fang Interactions of smoking with rs833061 polymorphism on the risk of non-alcoholic fat liver disease in Hubei Han population: a preliminary case–control study Iranian Journal of Basic Medical Sciences Interactions NAFLD Polymorphisms Smoking VEGF |
author_facet |
Pengbo Wu Yonglong Hua Shiyun Tan Ming Li Shu Yongxiang Guo Fang |
author_sort |
Pengbo Wu |
title |
Interactions of smoking with rs833061 polymorphism on the risk of non-alcoholic fat liver disease in Hubei Han population: a preliminary case–control study |
title_short |
Interactions of smoking with rs833061 polymorphism on the risk of non-alcoholic fat liver disease in Hubei Han population: a preliminary case–control study |
title_full |
Interactions of smoking with rs833061 polymorphism on the risk of non-alcoholic fat liver disease in Hubei Han population: a preliminary case–control study |
title_fullStr |
Interactions of smoking with rs833061 polymorphism on the risk of non-alcoholic fat liver disease in Hubei Han population: a preliminary case–control study |
title_full_unstemmed |
Interactions of smoking with rs833061 polymorphism on the risk of non-alcoholic fat liver disease in Hubei Han population: a preliminary case–control study |
title_sort |
interactions of smoking with rs833061 polymorphism on the risk of non-alcoholic fat liver disease in hubei han population: a preliminary case–control study |
publisher |
Mashhad University of Medical Sciences |
series |
Iranian Journal of Basic Medical Sciences |
issn |
2008-3866 2008-3874 |
publishDate |
2015-11-01 |
description |
Objective(s):Vascular endothelial growth factor (VEGF) has biological actions on energy homeostasis, inflammation and insulin resistance. The present study aimed to investigate the association between VEGF -460 T/C (rs833061), and +936 C/T (rs3025039) polymorphism and risk of non-alcohol fatty liver disease (NAFLD) in Hubei Han population and to further explore the interactions of smoking with rs833061 and rs3025039.
Materials and Methods:341 healthy controls and 246 cases were recruited. Two variants, rs833061 and rs3025039, were genotyped by polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP). The unconditional logistic regression (ULR) was performed to assess the association of the two variants with risk of NAFLD. Gene-environment interactions on the risk of NAFLD were preliminarily explored by generalized multifactor dimensionality reduction (GMDR) and further confirmed by ULR methods.
Results:After adjusting for covariates, increased risk of NAFLD was observed in patients carrying CT/CC genotypes in rs833061 and rs3025039 (ORa=1.80, 95% confidence interval (CI): 1.51, 2.36, Pa=0.000; ORa=1.89, 95% CI: 1.41, 2.82, Pa=0.000, respectively). Interaction of smoking with rs833061 was found by GMDR, with maximum prediction accuracy (67.91%) and a maximum cross-validation consistency (10/10). ULR method confirmed that, smoking-positive patients with genotype CT/CC had 4.93 times risk of NAFLD compared to smoking-negative participants with genotype TT (ORadda=4.93, 95% CI:2.91, 8.54, Padda=0.000), which further confirmed synergistic effects.
Conclusion: The results indicated that both rs833061 and rs3025039 are associated with NAFLD risk. Furthermore, rs833061 is likely to have an interaction with smoking, and they have synergistic effects on risk of NAFLD in Hubei Han population. |
topic |
Interactions NAFLD Polymorphisms Smoking VEGF |
url |
http://ijbms.mums.ac.ir/pdf_6050_c476d3dbd222b2a88d8864d537ea34da.html |
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