Interactions of smoking with rs833061 polymorphism on the risk of non-alcoholic fat liver disease in Hubei Han population: a preliminary case–control study

Objective(s):Vascular endothelial growth factor (VEGF) has biological actions on energy homeostasis, inflammation and insulin resistance. The present study aimed to investigate the association between VEGF -460 T/C (rs833061), and +936 C/T (rs3025039) polymorphism and risk of non-alcohol fatty liver...

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Main Authors: Pengbo Wu, Yonglong Hua, Shiyun Tan, Ming Li, Shu Yongxiang, Guo Fang
Format: Article
Language:English
Published: Mashhad University of Medical Sciences 2015-11-01
Series:Iranian Journal of Basic Medical Sciences
Subjects:
Online Access:http://ijbms.mums.ac.ir/pdf_6050_c476d3dbd222b2a88d8864d537ea34da.html
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spelling doaj-a51f54d1ef714f7896d7c3c2728b103b2020-11-24T21:01:27ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences 2008-38662008-38742015-11-011811111211176050Interactions of smoking with rs833061 polymorphism on the risk of non-alcoholic fat liver disease in Hubei Han population: a preliminary case–control studyPengbo Wu0Yonglong Hua1Shiyun Tan2Ming Li3Shu Yongxiang4Guo Fang5Department of Gastroenterology, Renmin Hospital of Wuhan University, Jiefang Road, Wuhan, ChinaDepartment of Gastroenterology, Renmin Hospital of Tongcheng, Minzhu Road 120, Hubei, ChinaDepartment of Gastroenterology, Renmin Hospital of Wuhan University, Jiefang Road, Wuhan, ChinaDepartment of Gastroenterology, Renmin Hospital of Wuhan University, Jiefang Road, Wuhan, ChinaDepartment of Gastroenterology, Renmin Hospital of Wuhan University, Jiefang Road, Wuhan, ChinaDepartment of Gastroenterology, Renmin Hospital of Wuhan University, Jiefang Road, Wuhan, ChinaObjective(s):Vascular endothelial growth factor (VEGF) has biological actions on energy homeostasis, inflammation and insulin resistance. The present study aimed to investigate the association between VEGF -460 T/C (rs833061), and +936 C/T (rs3025039) polymorphism and risk of non-alcohol fatty liver disease (NAFLD) in Hubei Han population and to further explore the interactions of smoking with rs833061 and rs3025039. Materials and Methods:341 healthy controls and 246 cases were recruited. Two variants, rs833061 and rs3025039, were genotyped by polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP). The unconditional logistic regression (ULR) was performed to assess the association of the two variants with risk of NAFLD. Gene-environment interactions on the risk of NAFLD were preliminarily explored by generalized multifactor dimensionality reduction (GMDR) and further confirmed by ULR methods. Results:After adjusting for covariates, increased risk of NAFLD was observed in patients carrying CT/CC genotypes in rs833061 and rs3025039 (ORa=1.80, 95% confidence interval (CI): 1.51, 2.36, Pa=0.000; ORa=1.89, 95% CI: 1.41, 2.82, Pa=0.000, respectively). Interaction of smoking with rs833061 was found by GMDR, with maximum prediction accuracy (67.91%) and a maximum cross-validation consistency (10/10). ULR method confirmed that, smoking-positive patients with genotype CT/CC had 4.93 times risk of NAFLD compared to smoking-negative participants with genotype TT (ORadda=4.93, 95% CI:2.91, 8.54, Padda=0.000), which further confirmed synergistic effects. Conclusion: The results indicated that both rs833061 and rs3025039 are associated with NAFLD risk. Furthermore, rs833061 is likely to have an interaction with smoking, and they have synergistic effects on risk of NAFLD in Hubei Han population.http://ijbms.mums.ac.ir/pdf_6050_c476d3dbd222b2a88d8864d537ea34da.htmlInteractionsNAFLDPolymorphismsSmokingVEGF
collection DOAJ
language English
format Article
sources DOAJ
author Pengbo Wu
Yonglong Hua
Shiyun Tan
Ming Li
Shu Yongxiang
Guo Fang
spellingShingle Pengbo Wu
Yonglong Hua
Shiyun Tan
Ming Li
Shu Yongxiang
Guo Fang
Interactions of smoking with rs833061 polymorphism on the risk of non-alcoholic fat liver disease in Hubei Han population: a preliminary case–control study
Iranian Journal of Basic Medical Sciences
Interactions
NAFLD
Polymorphisms
Smoking
VEGF
author_facet Pengbo Wu
Yonglong Hua
Shiyun Tan
Ming Li
Shu Yongxiang
Guo Fang
author_sort Pengbo Wu
title Interactions of smoking with rs833061 polymorphism on the risk of non-alcoholic fat liver disease in Hubei Han population: a preliminary case–control study
title_short Interactions of smoking with rs833061 polymorphism on the risk of non-alcoholic fat liver disease in Hubei Han population: a preliminary case–control study
title_full Interactions of smoking with rs833061 polymorphism on the risk of non-alcoholic fat liver disease in Hubei Han population: a preliminary case–control study
title_fullStr Interactions of smoking with rs833061 polymorphism on the risk of non-alcoholic fat liver disease in Hubei Han population: a preliminary case–control study
title_full_unstemmed Interactions of smoking with rs833061 polymorphism on the risk of non-alcoholic fat liver disease in Hubei Han population: a preliminary case–control study
title_sort interactions of smoking with rs833061 polymorphism on the risk of non-alcoholic fat liver disease in hubei han population: a preliminary case–control study
publisher Mashhad University of Medical Sciences
series Iranian Journal of Basic Medical Sciences
issn 2008-3866
2008-3874
publishDate 2015-11-01
description Objective(s):Vascular endothelial growth factor (VEGF) has biological actions on energy homeostasis, inflammation and insulin resistance. The present study aimed to investigate the association between VEGF -460 T/C (rs833061), and +936 C/T (rs3025039) polymorphism and risk of non-alcohol fatty liver disease (NAFLD) in Hubei Han population and to further explore the interactions of smoking with rs833061 and rs3025039. Materials and Methods:341 healthy controls and 246 cases were recruited. Two variants, rs833061 and rs3025039, were genotyped by polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP). The unconditional logistic regression (ULR) was performed to assess the association of the two variants with risk of NAFLD. Gene-environment interactions on the risk of NAFLD were preliminarily explored by generalized multifactor dimensionality reduction (GMDR) and further confirmed by ULR methods. Results:After adjusting for covariates, increased risk of NAFLD was observed in patients carrying CT/CC genotypes in rs833061 and rs3025039 (ORa=1.80, 95% confidence interval (CI): 1.51, 2.36, Pa=0.000; ORa=1.89, 95% CI: 1.41, 2.82, Pa=0.000, respectively). Interaction of smoking with rs833061 was found by GMDR, with maximum prediction accuracy (67.91%) and a maximum cross-validation consistency (10/10). ULR method confirmed that, smoking-positive patients with genotype CT/CC had 4.93 times risk of NAFLD compared to smoking-negative participants with genotype TT (ORadda=4.93, 95% CI:2.91, 8.54, Padda=0.000), which further confirmed synergistic effects. Conclusion: The results indicated that both rs833061 and rs3025039 are associated with NAFLD risk. Furthermore, rs833061 is likely to have an interaction with smoking, and they have synergistic effects on risk of NAFLD in Hubei Han population.
topic Interactions
NAFLD
Polymorphisms
Smoking
VEGF
url http://ijbms.mums.ac.ir/pdf_6050_c476d3dbd222b2a88d8864d537ea34da.html
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