A validation study of auditory function in an aminoglycoside-furosemide ototoxicity mouse model: Auditory brainstem response and distortion product otoacoustic emissions

A validation study of auditory function in an aminoglycoside-furosemide ototoxicity mouse model: Auditory brainstem response and distortion product otoacoustic emissions

Sensorineural hearing loss due to ototoxic drugs remains as a conflict as the treatment option with aminoglycosides. Ototoxic mouse model was produced with the administration of ototoxic drugs aminoglycoside kanamycin and loop-diuretic furosemide, thus validation of auditory function of the mouse mo...

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Main Authors: Yeji Ahn, Jin Sil Choi, Dae Hyun Kim, Temuulen Batsaikhan, Young Joon Seo
Format: Article
Language:English
Published: SAGE Publishing 2021-05-01
Series:Toxicology Research and Application
Online Access:https://doi.org/10.1177/23978473211016816
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spelling doaj-a51b174a5b184a79a9399676e20ea30d2021-05-13T03:03:28ZengSAGE PublishingToxicology Research and Application2397-84732021-05-01510.1177/23978473211016816A validation study of auditory function in an aminoglycoside-furosemide ototoxicity mouse model: Auditory brainstem response and distortion product otoacoustic emissionsYeji Ahn0Jin Sil Choi1Dae Hyun Kim2Temuulen Batsaikhan3Young Joon Seo4 Department of Otorhinolaryngology, Wonju College of Medicine, Yonsei University, Wonju, South Korea Department of Otorhinolaryngology, Wonju College of Medicine, Yonsei University, Wonju, South Korea Department of Medicine, Wonju College of Medicine, Yonsei University, Wonju, South Korea Department of Otorhinolaryngology, Wonju College of Medicine, Yonsei University, Wonju, South Korea Department of Otorhinolaryngology, Wonju College of Medicine, Yonsei University, Wonju, South KoreaSensorineural hearing loss due to ototoxic drugs remains as a conflict as the treatment option with aminoglycosides. Ototoxic mouse model was produced with the administration of ototoxic drugs aminoglycoside kanamycin and loop-diuretic furosemide, thus validation of auditory function of the mouse model is needed to determine the efficacy of the drugs. Kanamycin sulfate 550 mg/kg (VWR life sciences, PA, USA) and furosemide 130 mg/kg (Lasix, Handok, Korea) were administered through subcutaneous and intraperitoneal injection respectively. Auditory brainstem response and distortion otoacoustic emission tests were performed on days 3,5,7,10,14 post administration of the ototoxic drug. Thresholds in response to the stimulus given in the auditory brainstem recordings and distortion otoacoustic emission tests were obtained. The hearing threshold shift to high stimulus intensity was observed post administration of the ototoxic drug. Latency of the ABR peak waves were recorded and analyzed, latency delay was observed as hearing threshold increases. These findings will further support in the application of this animal model in various studies regarding ototoxic hearing loss.https://doi.org/10.1177/23978473211016816
collection DOAJ
language English
format Article
sources DOAJ
author Yeji Ahn
Jin Sil Choi
Dae Hyun Kim
Temuulen Batsaikhan
Young Joon Seo
spellingShingle Yeji Ahn
Jin Sil Choi
Dae Hyun Kim
Temuulen Batsaikhan
Young Joon Seo
A validation study of auditory function in an aminoglycoside-furosemide ototoxicity mouse model: Auditory brainstem response and distortion product otoacoustic emissions
Toxicology Research and Application
author_facet Yeji Ahn
Jin Sil Choi
Dae Hyun Kim
Temuulen Batsaikhan
Young Joon Seo
author_sort Yeji Ahn
title A validation study of auditory function in an aminoglycoside-furosemide ototoxicity mouse model: Auditory brainstem response and distortion product otoacoustic emissions
title_short A validation study of auditory function in an aminoglycoside-furosemide ototoxicity mouse model: Auditory brainstem response and distortion product otoacoustic emissions
title_full A validation study of auditory function in an aminoglycoside-furosemide ototoxicity mouse model: Auditory brainstem response and distortion product otoacoustic emissions
title_fullStr A validation study of auditory function in an aminoglycoside-furosemide ototoxicity mouse model: Auditory brainstem response and distortion product otoacoustic emissions
title_full_unstemmed A validation study of auditory function in an aminoglycoside-furosemide ototoxicity mouse model: Auditory brainstem response and distortion product otoacoustic emissions
title_sort validation study of auditory function in an aminoglycoside-furosemide ototoxicity mouse model: auditory brainstem response and distortion product otoacoustic emissions
publisher SAGE Publishing
series Toxicology Research and Application
issn 2397-8473
publishDate 2021-05-01
description Sensorineural hearing loss due to ototoxic drugs remains as a conflict as the treatment option with aminoglycosides. Ototoxic mouse model was produced with the administration of ototoxic drugs aminoglycoside kanamycin and loop-diuretic furosemide, thus validation of auditory function of the mouse model is needed to determine the efficacy of the drugs. Kanamycin sulfate 550 mg/kg (VWR life sciences, PA, USA) and furosemide 130 mg/kg (Lasix, Handok, Korea) were administered through subcutaneous and intraperitoneal injection respectively. Auditory brainstem response and distortion otoacoustic emission tests were performed on days 3,5,7,10,14 post administration of the ototoxic drug. Thresholds in response to the stimulus given in the auditory brainstem recordings and distortion otoacoustic emission tests were obtained. The hearing threshold shift to high stimulus intensity was observed post administration of the ototoxic drug. Latency of the ABR peak waves were recorded and analyzed, latency delay was observed as hearing threshold increases. These findings will further support in the application of this animal model in various studies regarding ototoxic hearing loss.
url https://doi.org/10.1177/23978473211016816
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