Self-reactivities to the non-erythroid alpha spectrin correlate with cerebral malaria in Gabonese children.

<h4>Background</h4>Hypergammaglobulinemia and polyclonal B-cell activation commonly occur in Plasmodium sp. infections. Some of the antibodies produced recognize self-components and are correlated with disease severity in P. falciparum malaria. However, it is not known whether some self-...

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Main Authors: Vincent Guiyedi, Youri Chanseaud, Constantin Fesel, Georges Snounou, Jean-Claude Rousselle, Pharat Lim, Jean Koko, Abdelkader Namane, Pierre-André Cazenave, Maryvonne Kombila, Sylviane Pied
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2007-04-01
Series:PLoS ONE
Online Access:https://doi.org/10.1371/journal.pone.0000389
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spelling doaj-a506ac4fe2e642c7b589484d6cf0d3462021-03-03T22:29:19ZengPublic Library of Science (PLoS)PLoS ONE1932-62032007-04-0124e38910.1371/journal.pone.0000389Self-reactivities to the non-erythroid alpha spectrin correlate with cerebral malaria in Gabonese children.Vincent GuiyediYouri ChanseaudConstantin FeselGeorges SnounouJean-Claude RoussellePharat LimJean KokoAbdelkader NamanePierre-André CazenaveMaryvonne KombilaSylviane Pied<h4>Background</h4>Hypergammaglobulinemia and polyclonal B-cell activation commonly occur in Plasmodium sp. infections. Some of the antibodies produced recognize self-components and are correlated with disease severity in P. falciparum malaria. However, it is not known whether some self-reactive antibodies produced during P. falciparum infection contribute to the events leading to cerebral malaria (CM). We show here a correlation between self-antibody responses to a human brain protein and high levels of circulating TNF alpha (TNFalpha), with the manifestation of CM in Gabonese children.<h4>Methodology</h4>To study the role of self-reactive antibodies associated to the development of P. falciparum cerebral malaria, we used a combination of quantitative immunoblotting and multivariate analysis to analyse correlation between the reactivity of circulating IgG with a human brain protein extract and TNFalpha concentrations in cohorts of uninfected controls (UI) and P. falciparum-infected Gabonese children developing uncomplicated malaria (UM), severe non-cerebral malaria (SNCM), or CM.<h4>Results/conclusion</h4>The repertoire of brain antigens recognized by plasma IgGs was more diverse in infected than in UI individuals. Anti-brain reactivity was significantly higher in the CM group than in the UM and SNCM groups. IgG self-reactivity to brain antigens was also correlated with plasma IgG levels and age. We found that 90% of CM patients displayed reactivity to a high-molecular mass band containing the spectrin non-erythroid alpha chain. Reactivity with this band was correlated with high TNFalpha concentrations in CM patients. These results strongly suggest that an antibody response to brain antigens induced by P. falciparum infection may be associated with pathogenic mechanisms in patients developing CM.https://doi.org/10.1371/journal.pone.0000389
collection DOAJ
language English
format Article
sources DOAJ
author Vincent Guiyedi
Youri Chanseaud
Constantin Fesel
Georges Snounou
Jean-Claude Rousselle
Pharat Lim
Jean Koko
Abdelkader Namane
Pierre-André Cazenave
Maryvonne Kombila
Sylviane Pied
spellingShingle Vincent Guiyedi
Youri Chanseaud
Constantin Fesel
Georges Snounou
Jean-Claude Rousselle
Pharat Lim
Jean Koko
Abdelkader Namane
Pierre-André Cazenave
Maryvonne Kombila
Sylviane Pied
Self-reactivities to the non-erythroid alpha spectrin correlate with cerebral malaria in Gabonese children.
PLoS ONE
author_facet Vincent Guiyedi
Youri Chanseaud
Constantin Fesel
Georges Snounou
Jean-Claude Rousselle
Pharat Lim
Jean Koko
Abdelkader Namane
Pierre-André Cazenave
Maryvonne Kombila
Sylviane Pied
author_sort Vincent Guiyedi
title Self-reactivities to the non-erythroid alpha spectrin correlate with cerebral malaria in Gabonese children.
title_short Self-reactivities to the non-erythroid alpha spectrin correlate with cerebral malaria in Gabonese children.
title_full Self-reactivities to the non-erythroid alpha spectrin correlate with cerebral malaria in Gabonese children.
title_fullStr Self-reactivities to the non-erythroid alpha spectrin correlate with cerebral malaria in Gabonese children.
title_full_unstemmed Self-reactivities to the non-erythroid alpha spectrin correlate with cerebral malaria in Gabonese children.
title_sort self-reactivities to the non-erythroid alpha spectrin correlate with cerebral malaria in gabonese children.
publisher Public Library of Science (PLoS)
series PLoS ONE
issn 1932-6203
publishDate 2007-04-01
description <h4>Background</h4>Hypergammaglobulinemia and polyclonal B-cell activation commonly occur in Plasmodium sp. infections. Some of the antibodies produced recognize self-components and are correlated with disease severity in P. falciparum malaria. However, it is not known whether some self-reactive antibodies produced during P. falciparum infection contribute to the events leading to cerebral malaria (CM). We show here a correlation between self-antibody responses to a human brain protein and high levels of circulating TNF alpha (TNFalpha), with the manifestation of CM in Gabonese children.<h4>Methodology</h4>To study the role of self-reactive antibodies associated to the development of P. falciparum cerebral malaria, we used a combination of quantitative immunoblotting and multivariate analysis to analyse correlation between the reactivity of circulating IgG with a human brain protein extract and TNFalpha concentrations in cohorts of uninfected controls (UI) and P. falciparum-infected Gabonese children developing uncomplicated malaria (UM), severe non-cerebral malaria (SNCM), or CM.<h4>Results/conclusion</h4>The repertoire of brain antigens recognized by plasma IgGs was more diverse in infected than in UI individuals. Anti-brain reactivity was significantly higher in the CM group than in the UM and SNCM groups. IgG self-reactivity to brain antigens was also correlated with plasma IgG levels and age. We found that 90% of CM patients displayed reactivity to a high-molecular mass band containing the spectrin non-erythroid alpha chain. Reactivity with this band was correlated with high TNFalpha concentrations in CM patients. These results strongly suggest that an antibody response to brain antigens induced by P. falciparum infection may be associated with pathogenic mechanisms in patients developing CM.
url https://doi.org/10.1371/journal.pone.0000389
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