Self-reactivities to the non-erythroid alpha spectrin correlate with cerebral malaria in Gabonese children.
<h4>Background</h4>Hypergammaglobulinemia and polyclonal B-cell activation commonly occur in Plasmodium sp. infections. Some of the antibodies produced recognize self-components and are correlated with disease severity in P. falciparum malaria. However, it is not known whether some self-...
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doaj-a506ac4fe2e642c7b589484d6cf0d3462021-03-03T22:29:19ZengPublic Library of Science (PLoS)PLoS ONE1932-62032007-04-0124e38910.1371/journal.pone.0000389Self-reactivities to the non-erythroid alpha spectrin correlate with cerebral malaria in Gabonese children.Vincent GuiyediYouri ChanseaudConstantin FeselGeorges SnounouJean-Claude RoussellePharat LimJean KokoAbdelkader NamanePierre-André CazenaveMaryvonne KombilaSylviane Pied<h4>Background</h4>Hypergammaglobulinemia and polyclonal B-cell activation commonly occur in Plasmodium sp. infections. Some of the antibodies produced recognize self-components and are correlated with disease severity in P. falciparum malaria. However, it is not known whether some self-reactive antibodies produced during P. falciparum infection contribute to the events leading to cerebral malaria (CM). We show here a correlation between self-antibody responses to a human brain protein and high levels of circulating TNF alpha (TNFalpha), with the manifestation of CM in Gabonese children.<h4>Methodology</h4>To study the role of self-reactive antibodies associated to the development of P. falciparum cerebral malaria, we used a combination of quantitative immunoblotting and multivariate analysis to analyse correlation between the reactivity of circulating IgG with a human brain protein extract and TNFalpha concentrations in cohorts of uninfected controls (UI) and P. falciparum-infected Gabonese children developing uncomplicated malaria (UM), severe non-cerebral malaria (SNCM), or CM.<h4>Results/conclusion</h4>The repertoire of brain antigens recognized by plasma IgGs was more diverse in infected than in UI individuals. Anti-brain reactivity was significantly higher in the CM group than in the UM and SNCM groups. IgG self-reactivity to brain antigens was also correlated with plasma IgG levels and age. We found that 90% of CM patients displayed reactivity to a high-molecular mass band containing the spectrin non-erythroid alpha chain. Reactivity with this band was correlated with high TNFalpha concentrations in CM patients. These results strongly suggest that an antibody response to brain antigens induced by P. falciparum infection may be associated with pathogenic mechanisms in patients developing CM.https://doi.org/10.1371/journal.pone.0000389 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Vincent Guiyedi Youri Chanseaud Constantin Fesel Georges Snounou Jean-Claude Rousselle Pharat Lim Jean Koko Abdelkader Namane Pierre-André Cazenave Maryvonne Kombila Sylviane Pied |
spellingShingle |
Vincent Guiyedi Youri Chanseaud Constantin Fesel Georges Snounou Jean-Claude Rousselle Pharat Lim Jean Koko Abdelkader Namane Pierre-André Cazenave Maryvonne Kombila Sylviane Pied Self-reactivities to the non-erythroid alpha spectrin correlate with cerebral malaria in Gabonese children. PLoS ONE |
author_facet |
Vincent Guiyedi Youri Chanseaud Constantin Fesel Georges Snounou Jean-Claude Rousselle Pharat Lim Jean Koko Abdelkader Namane Pierre-André Cazenave Maryvonne Kombila Sylviane Pied |
author_sort |
Vincent Guiyedi |
title |
Self-reactivities to the non-erythroid alpha spectrin correlate with cerebral malaria in Gabonese children. |
title_short |
Self-reactivities to the non-erythroid alpha spectrin correlate with cerebral malaria in Gabonese children. |
title_full |
Self-reactivities to the non-erythroid alpha spectrin correlate with cerebral malaria in Gabonese children. |
title_fullStr |
Self-reactivities to the non-erythroid alpha spectrin correlate with cerebral malaria in Gabonese children. |
title_full_unstemmed |
Self-reactivities to the non-erythroid alpha spectrin correlate with cerebral malaria in Gabonese children. |
title_sort |
self-reactivities to the non-erythroid alpha spectrin correlate with cerebral malaria in gabonese children. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2007-04-01 |
description |
<h4>Background</h4>Hypergammaglobulinemia and polyclonal B-cell activation commonly occur in Plasmodium sp. infections. Some of the antibodies produced recognize self-components and are correlated with disease severity in P. falciparum malaria. However, it is not known whether some self-reactive antibodies produced during P. falciparum infection contribute to the events leading to cerebral malaria (CM). We show here a correlation between self-antibody responses to a human brain protein and high levels of circulating TNF alpha (TNFalpha), with the manifestation of CM in Gabonese children.<h4>Methodology</h4>To study the role of self-reactive antibodies associated to the development of P. falciparum cerebral malaria, we used a combination of quantitative immunoblotting and multivariate analysis to analyse correlation between the reactivity of circulating IgG with a human brain protein extract and TNFalpha concentrations in cohorts of uninfected controls (UI) and P. falciparum-infected Gabonese children developing uncomplicated malaria (UM), severe non-cerebral malaria (SNCM), or CM.<h4>Results/conclusion</h4>The repertoire of brain antigens recognized by plasma IgGs was more diverse in infected than in UI individuals. Anti-brain reactivity was significantly higher in the CM group than in the UM and SNCM groups. IgG self-reactivity to brain antigens was also correlated with plasma IgG levels and age. We found that 90% of CM patients displayed reactivity to a high-molecular mass band containing the spectrin non-erythroid alpha chain. Reactivity with this band was correlated with high TNFalpha concentrations in CM patients. These results strongly suggest that an antibody response to brain antigens induced by P. falciparum infection may be associated with pathogenic mechanisms in patients developing CM. |
url |
https://doi.org/10.1371/journal.pone.0000389 |
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