Plant Milking Technology—An Innovative and Sustainable Process to Produce Highly Active Extracts from Plant Roots
We have used an original technology (Plant Milking Technology) based on aeroponic cultivation of plants associated with the gentle recovery of active ingredients from roots. Extraction of bioactive molecules was achieved by soaking the roots, still attached to the living plants, into a nontoxic solv...
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doaj-a4e44eff8c5a4e228dbdedc86bd45fa42020-11-25T03:21:57ZengMDPI AGMolecules1420-30492020-09-01254162416210.3390/molecules25184162Plant Milking Technology—An Innovative and Sustainable Process to Produce Highly Active Extracts from Plant RootsHanane Chajra0Aleksander Salwinski1Agnès Guillaumin2Benoit Mignard3Paul Hannewald4Léonor Duriot5Pierre Warnault6Carine Guillet-Claude7Mathilde Fréchet8Frédéric Bourgaud9Clariant Active Ingredients, 195 route d′Espagne, 31000 Toulouse, FrancePlant Advanced Technologies, 19 Avenue de la Forêt de Haye, 54500 Vandoeuvre, FrancePlant Advanced Technologies, 19 Avenue de la Forêt de Haye, 54500 Vandoeuvre, FranceClariant Active Ingredients, 195 route d′Espagne, 31000 Toulouse, FrancePlant Advanced Technologies, 19 Avenue de la Forêt de Haye, 54500 Vandoeuvre, FrancePlant Advanced Technologies, 19 Avenue de la Forêt de Haye, 54500 Vandoeuvre, FrancePlant Advanced Technologies, 19 Avenue de la Forêt de Haye, 54500 Vandoeuvre, FrancePlant Advanced Technologies, 19 Avenue de la Forêt de Haye, 54500 Vandoeuvre, FranceClariant Active Ingredients, 195 route d′Espagne, 31000 Toulouse, FrancePlant Advanced Technologies, 19 Avenue de la Forêt de Haye, 54500 Vandoeuvre, FranceWe have used an original technology (Plant Milking Technology) based on aeroponic cultivation of plants associated with the gentle recovery of active ingredients from roots. Extraction of bioactive molecules was achieved by soaking the roots, still attached to the living plants, into a nontoxic solvent for a 2 h period. This nondestructive recovery process allows using the same root biomass for successive harvesting dates, in a recyclable way. We have applied this technology to <i>Morus alba</i> L. (mulberry tree), an emblematic tree of the Traditional Chinese Medicine (TCM). Trees were aeroponically grown in large-scale devices (100 m<sup>2</sup>) and were submitted to nitrogen deprivation to increase the content in active molecules (prenylated flavonoids). The Plant Milking technology applied to <i>Morus alba</i> L. allowed to produce an extract enriched in prenylated compounds (18-fold increase when compared to commercial root extract). Prenylated flavonoids (moracenin A and B, kuwanon C, wittiorumin F, morusin) presented a high affinity for the aged-associated collagenase enzyme, which was confirmed by activity inhibition. In accordance, <i>M. alba</i> extract presents efficient properties to regulate the skin matrisome, which is critical during skin aging. The benefits have been especially confirmed in vivo on wrinkle reduction, in a clinical study that involved aged women. Plant Milking technology is an optimal solution to produce active ingredients from plant roots, including trees, that meet both customer expectations around sustainability, as well as the need for an efficient production system for biotechnologists.https://www.mdpi.com/1420-3049/25/18/4162natural compoundrootsustainabilitymoracenin<i>Morus alba </i>L.prenylated flavonoids |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hanane Chajra Aleksander Salwinski Agnès Guillaumin Benoit Mignard Paul Hannewald Léonor Duriot Pierre Warnault Carine Guillet-Claude Mathilde Fréchet Frédéric Bourgaud |
spellingShingle |
Hanane Chajra Aleksander Salwinski Agnès Guillaumin Benoit Mignard Paul Hannewald Léonor Duriot Pierre Warnault Carine Guillet-Claude Mathilde Fréchet Frédéric Bourgaud Plant Milking Technology—An Innovative and Sustainable Process to Produce Highly Active Extracts from Plant Roots Molecules natural compound root sustainability moracenin <i>Morus alba </i>L. prenylated flavonoids |
author_facet |
Hanane Chajra Aleksander Salwinski Agnès Guillaumin Benoit Mignard Paul Hannewald Léonor Duriot Pierre Warnault Carine Guillet-Claude Mathilde Fréchet Frédéric Bourgaud |
author_sort |
Hanane Chajra |
title |
Plant Milking Technology—An Innovative and Sustainable Process to Produce Highly Active Extracts from Plant Roots |
title_short |
Plant Milking Technology—An Innovative and Sustainable Process to Produce Highly Active Extracts from Plant Roots |
title_full |
Plant Milking Technology—An Innovative and Sustainable Process to Produce Highly Active Extracts from Plant Roots |
title_fullStr |
Plant Milking Technology—An Innovative and Sustainable Process to Produce Highly Active Extracts from Plant Roots |
title_full_unstemmed |
Plant Milking Technology—An Innovative and Sustainable Process to Produce Highly Active Extracts from Plant Roots |
title_sort |
plant milking technology—an innovative and sustainable process to produce highly active extracts from plant roots |
publisher |
MDPI AG |
series |
Molecules |
issn |
1420-3049 |
publishDate |
2020-09-01 |
description |
We have used an original technology (Plant Milking Technology) based on aeroponic cultivation of plants associated with the gentle recovery of active ingredients from roots. Extraction of bioactive molecules was achieved by soaking the roots, still attached to the living plants, into a nontoxic solvent for a 2 h period. This nondestructive recovery process allows using the same root biomass for successive harvesting dates, in a recyclable way. We have applied this technology to <i>Morus alba</i> L. (mulberry tree), an emblematic tree of the Traditional Chinese Medicine (TCM). Trees were aeroponically grown in large-scale devices (100 m<sup>2</sup>) and were submitted to nitrogen deprivation to increase the content in active molecules (prenylated flavonoids). The Plant Milking technology applied to <i>Morus alba</i> L. allowed to produce an extract enriched in prenylated compounds (18-fold increase when compared to commercial root extract). Prenylated flavonoids (moracenin A and B, kuwanon C, wittiorumin F, morusin) presented a high affinity for the aged-associated collagenase enzyme, which was confirmed by activity inhibition. In accordance, <i>M. alba</i> extract presents efficient properties to regulate the skin matrisome, which is critical during skin aging. The benefits have been especially confirmed in vivo on wrinkle reduction, in a clinical study that involved aged women. Plant Milking technology is an optimal solution to produce active ingredients from plant roots, including trees, that meet both customer expectations around sustainability, as well as the need for an efficient production system for biotechnologists. |
topic |
natural compound root sustainability moracenin <i>Morus alba </i>L. prenylated flavonoids |
url |
https://www.mdpi.com/1420-3049/25/18/4162 |
work_keys_str_mv |
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