Protease Substrate-Independent Universal Assay for Monitoring Digestion of Native Unmodified Proteins

Protease Substrate-Independent Universal Assay for Monitoring Digestion of Native Unmodified Proteins

Proteases are a group of enzymes with a catalytic function to hydrolyze peptide bonds of proteins. Proteases regulate the activity, signaling mechanism, fate, and localization of many proteins, and their dysregulation is associated with various pathological conditions. Proteases have been identified...

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Bibliographic Details
Main Authors: Emmiliisa Vuorinen, Salla Valtonen, Nazia Hassan, Randa Mahran, Huda Habib, Morteza Malakoutikhah, Kari Kopra, Harri Härmä
Format: Article
Language:English
Published: MDPI AG 2021-06-01
Series:International Journal of Molecular Sciences
Subjects:
protease activity
protease inhibition
digestion
time-resolved luminescence
label-free
Online Access:https://www.mdpi.com/1422-0067/22/12/6362

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