The structural dynamics of macropinosome formation and PI3-kinase-mediated sealing revealed by lattice light sheet microscopy

The structural dynamics of macropinosome formation and PI3-kinase-mediated sealing revealed by lattice light sheet microscopy

Macropinocytosis is a cellular process for the uptake of extracellular fluid. Here, the authors use lattice light sheet microscopy to examine the spatial dynamics of the plasma membrane, PI3K activity, and structural differences of various macrophage cell types during macropinocytosis.

Bibliographic Details
Main Authors: Shayne E. Quinn, Lu Huang, Jason G. Kerkvliet, Joel A. Swanson, Steve Smith, Adam D. Hoppe, Robert B. Anderson, Natalie W. Thiex, Brandon L. Scott
Format: Article
Language:English
Published: Nature Publishing Group 2021-08-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-021-25187-1
id doaj-a4d0a54149e7410e8dfc5feb28705c33
record_format Article
spelling doaj-a4d0a54149e7410e8dfc5feb28705c332021-08-15T11:39:02ZengNature Publishing GroupNature Communications2041-17232021-08-0112111210.1038/s41467-021-25187-1The structural dynamics of macropinosome formation and PI3-kinase-mediated sealing revealed by lattice light sheet microscopyShayne E. Quinn0Lu Huang1Jason G. Kerkvliet2Joel A. Swanson3Steve Smith4Adam D. Hoppe5Robert B. Anderson6Natalie W. Thiex7Brandon L. Scott8Nanoscience and Nanoengineering, South Dakota School of Mines and Technology (South Dakota Mines)Department of Biology and Microbiology, South Dakota State University (SDSU)BioSNTR, SDSUDepartment of Microbiology and Immunology, University of MichiganNanoscience and Nanoengineering, South Dakota School of Mines and Technology (South Dakota Mines)BioSNTR, SDSUNanoscience and Nanoengineering, South Dakota School of Mines and Technology (South Dakota Mines)Department of Biology and Microbiology, South Dakota State University (SDSU)Nanoscience and Nanoengineering, South Dakota School of Mines and Technology (South Dakota Mines)Macropinocytosis is a cellular process for the uptake of extracellular fluid. Here, the authors use lattice light sheet microscopy to examine the spatial dynamics of the plasma membrane, PI3K activity, and structural differences of various macrophage cell types during macropinocytosis.https://doi.org/10.1038/s41467-021-25187-1
collection DOAJ
language English
format Article
sources DOAJ
author Shayne E. Quinn
Lu Huang
Jason G. Kerkvliet
Joel A. Swanson
Steve Smith
Adam D. Hoppe
Robert B. Anderson
Natalie W. Thiex
Brandon L. Scott
spellingShingle Shayne E. Quinn
Lu Huang
Jason G. Kerkvliet
Joel A. Swanson
Steve Smith
Adam D. Hoppe
Robert B. Anderson
Natalie W. Thiex
Brandon L. Scott
The structural dynamics of macropinosome formation and PI3-kinase-mediated sealing revealed by lattice light sheet microscopy
Nature Communications
author_facet Shayne E. Quinn
Lu Huang
Jason G. Kerkvliet
Joel A. Swanson
Steve Smith
Adam D. Hoppe
Robert B. Anderson
Natalie W. Thiex
Brandon L. Scott
author_sort Shayne E. Quinn
title The structural dynamics of macropinosome formation and PI3-kinase-mediated sealing revealed by lattice light sheet microscopy
title_short The structural dynamics of macropinosome formation and PI3-kinase-mediated sealing revealed by lattice light sheet microscopy
title_full The structural dynamics of macropinosome formation and PI3-kinase-mediated sealing revealed by lattice light sheet microscopy
title_fullStr The structural dynamics of macropinosome formation and PI3-kinase-mediated sealing revealed by lattice light sheet microscopy
title_full_unstemmed The structural dynamics of macropinosome formation and PI3-kinase-mediated sealing revealed by lattice light sheet microscopy
title_sort structural dynamics of macropinosome formation and pi3-kinase-mediated sealing revealed by lattice light sheet microscopy
publisher Nature Publishing Group
series Nature Communications
issn 2041-1723
publishDate 2021-08-01
description Macropinocytosis is a cellular process for the uptake of extracellular fluid. Here, the authors use lattice light sheet microscopy to examine the spatial dynamics of the plasma membrane, PI3K activity, and structural differences of various macrophage cell types during macropinocytosis.
url https://doi.org/10.1038/s41467-021-25187-1
work_keys_str_mv AT shayneequinn thestructuraldynamicsofmacropinosomeformationandpi3kinasemediatedsealingrevealedbylatticelightsheetmicroscopy
AT luhuang thestructuraldynamicsofmacropinosomeformationandpi3kinasemediatedsealingrevealedbylatticelightsheetmicroscopy
AT jasongkerkvliet thestructuraldynamicsofmacropinosomeformationandpi3kinasemediatedsealingrevealedbylatticelightsheetmicroscopy
AT joelaswanson thestructuraldynamicsofmacropinosomeformationandpi3kinasemediatedsealingrevealedbylatticelightsheetmicroscopy
AT stevesmith thestructuraldynamicsofmacropinosomeformationandpi3kinasemediatedsealingrevealedbylatticelightsheetmicroscopy
AT adamdhoppe thestructuraldynamicsofmacropinosomeformationandpi3kinasemediatedsealingrevealedbylatticelightsheetmicroscopy
AT robertbanderson thestructuraldynamicsofmacropinosomeformationandpi3kinasemediatedsealingrevealedbylatticelightsheetmicroscopy
AT nataliewthiex thestructuraldynamicsofmacropinosomeformationandpi3kinasemediatedsealingrevealedbylatticelightsheetmicroscopy
AT brandonlscott thestructuraldynamicsofmacropinosomeformationandpi3kinasemediatedsealingrevealedbylatticelightsheetmicroscopy
AT shayneequinn structuraldynamicsofmacropinosomeformationandpi3kinasemediatedsealingrevealedbylatticelightsheetmicroscopy
AT luhuang structuraldynamicsofmacropinosomeformationandpi3kinasemediatedsealingrevealedbylatticelightsheetmicroscopy
AT jasongkerkvliet structuraldynamicsofmacropinosomeformationandpi3kinasemediatedsealingrevealedbylatticelightsheetmicroscopy
AT joelaswanson structuraldynamicsofmacropinosomeformationandpi3kinasemediatedsealingrevealedbylatticelightsheetmicroscopy
AT stevesmith structuraldynamicsofmacropinosomeformationandpi3kinasemediatedsealingrevealedbylatticelightsheetmicroscopy
AT adamdhoppe structuraldynamicsofmacropinosomeformationandpi3kinasemediatedsealingrevealedbylatticelightsheetmicroscopy
AT robertbanderson structuraldynamicsofmacropinosomeformationandpi3kinasemediatedsealingrevealedbylatticelightsheetmicroscopy
AT nataliewthiex structuraldynamicsofmacropinosomeformationandpi3kinasemediatedsealingrevealedbylatticelightsheetmicroscopy
AT brandonlscott structuraldynamicsofmacropinosomeformationandpi3kinasemediatedsealingrevealedbylatticelightsheetmicroscopy
_version_ 1721206556148629504

Similar Items