Telomere length assessments of muscle stem cells in rodent and human skeletal muscle sections

Telomere length assessments of muscle stem cells in rodent and human skeletal muscle sections

Summary: Measurements of telomere length in skeletal muscle stem cells (MuSCs), a rare cell population within muscles, provide insights into cellular dysfunction in diseased conditions. Here, we describe a protocol (cryosection muscle quantitative fluorescent in situ hybridization) using skeletal mu...

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Main Authors: Elisia D. Tichy, Foteini Mourkioti
Format: Article
Language:English
Published: Elsevier 2021-12-01
Series:STAR Protocols
Subjects:
Cell Biology
Cell isolation
Cell-based Assays
In Situ Hybridization
Microscopy
Stem Cells
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721005360
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spelling doaj-a4c73a2159ae49bbb4c91c7d49a12b082021-09-15T04:23:21ZengElsevierSTAR Protocols2666-16672021-12-0124100830Telomere length assessments of muscle stem cells in rodent and human skeletal muscle sectionsElisia D. Tichy0Foteini Mourkioti1Department of Orthopaedic Surgery, Perelman School of Medicine, The University of Pennsylvania, Philadelphia, PA 19104, USA; Corresponding authorDepartment of Orthopaedic Surgery, Perelman School of Medicine, The University of Pennsylvania, Philadelphia, PA 19104, USA; Department of Cell and Developmental Biology, Perelman School of Medicine, The University of Pennsylvania, Philadelphia, PA 19104, USA; Institute of Regenerative Medicine, Musculoskeletal Regeneration Program, Perelman School of Medicine, The University of Pennsylvania, Philadelphia, PA 19104, USA; Corresponding authorSummary: Measurements of telomere length in skeletal muscle stem cells (MuSCs), a rare cell population within muscles, provide insights into cellular dysfunction in diseased conditions. Here, we describe a protocol (cryosection muscle quantitative fluorescent in situ hybridization) using skeletal muscle cryosections for assessments of telomere length in MuSCs, in their native environment. Using a free software, telomere length measurements are assessed on a single-cell level. We also provide methodology to perform data analyses in several ways.For complete details on the use and execution of this protocol, please refer to Tichy et al. (2021).http://www.sciencedirect.com/science/article/pii/S2666166721005360Cell BiologyCell isolationCell-based AssaysIn Situ HybridizationMicroscopyStem Cells
collection DOAJ
language English
format Article
sources DOAJ
author Elisia D. Tichy
Foteini Mourkioti
spellingShingle Elisia D. Tichy
Foteini Mourkioti
Telomere length assessments of muscle stem cells in rodent and human skeletal muscle sections
STAR Protocols
Cell Biology
Cell isolation
Cell-based Assays
In Situ Hybridization
Microscopy
Stem Cells
author_facet Elisia D. Tichy
Foteini Mourkioti
author_sort Elisia D. Tichy
title Telomere length assessments of muscle stem cells in rodent and human skeletal muscle sections
title_short Telomere length assessments of muscle stem cells in rodent and human skeletal muscle sections
title_full Telomere length assessments of muscle stem cells in rodent and human skeletal muscle sections
title_fullStr Telomere length assessments of muscle stem cells in rodent and human skeletal muscle sections
title_full_unstemmed Telomere length assessments of muscle stem cells in rodent and human skeletal muscle sections
title_sort telomere length assessments of muscle stem cells in rodent and human skeletal muscle sections
publisher Elsevier
series STAR Protocols
issn 2666-1667
publishDate 2021-12-01
description Summary: Measurements of telomere length in skeletal muscle stem cells (MuSCs), a rare cell population within muscles, provide insights into cellular dysfunction in diseased conditions. Here, we describe a protocol (cryosection muscle quantitative fluorescent in situ hybridization) using skeletal muscle cryosections for assessments of telomere length in MuSCs, in their native environment. Using a free software, telomere length measurements are assessed on a single-cell level. We also provide methodology to perform data analyses in several ways.For complete details on the use and execution of this protocol, please refer to Tichy et al. (2021).
topic Cell Biology
Cell isolation
Cell-based Assays
In Situ Hybridization
Microscopy
Stem Cells
url http://www.sciencedirect.com/science/article/pii/S2666166721005360
work_keys_str_mv AT elisiadtichy telomerelengthassessmentsofmusclestemcellsinrodentandhumanskeletalmusclesections
AT foteinimourkioti telomerelengthassessmentsofmusclestemcellsinrodentandhumanskeletalmusclesections
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