An antigen microarray protocol for COVID-19 serological analysis

An antigen microarray protocol for COVID-19 serological analysis

Summary: The emergence of the coronavirus disease 2019 pandemic increased the interest in analysis of immunoglobulin responses. ELISA and lateral flow assays are widely used but are restricted by a single response value to an antigen or antigen pool. Here, we describe antigen microarrays, an alterna...

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Bibliographic Details
Main Authors: Joseph Longworth, Gunnar Dittmar
Format: Article
Language:English
Published: Elsevier 2021-09-01
Series:STAR Protocols
Subjects:
Antibody
Bioinformatics
Health Sciences
High Throughput Screening
Immunology
Proteomics
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721005219
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spelling doaj-a4bcd274e37b4800bfbd13b11f4f51d62021-09-19T05:01:27ZengElsevierSTAR Protocols2666-16672021-09-0123100815An antigen microarray protocol for COVID-19 serological analysisJoseph Longworth0Gunnar Dittmar1Luxembourg Institute of Health, RPPA Platform, 1A-B, rue Thomas Edison, 1445 Strassen, Luxembourg; Proteomics of Cellular Signaling, Luxembourg of Health, 1A, Rue Thomas Edison, 1445 Strassen, Luxembourg; Corresponding authorProteomics of Cellular Signaling, Luxembourg of Health, 1A, Rue Thomas Edison, 1445 Strassen, Luxembourg; Department of Life Sciences and Medicine, University of Luxembourg, 4367 Belvaux, Luxembourg; Corresponding authorSummary: The emergence of the coronavirus disease 2019 pandemic increased the interest in analysis of immunoglobulin responses. ELISA and lateral flow assays are widely used but are restricted by a single response value to an antigen or antigen pool. Here, we describe antigen microarrays, an alternative allowing simultaneous assessment of multiple interactions between antigens and the immunoglobulin content of patient sera. The technique requires minimal reagents and sample input and can be adapted to a wide variety of potential antigenic targets of interest.http://www.sciencedirect.com/science/article/pii/S2666166721005219AntibodyBioinformaticsHealth SciencesHigh Throughput ScreeningImmunologyProteomics
collection DOAJ
language English
format Article
sources DOAJ
author Joseph Longworth
Gunnar Dittmar
spellingShingle Joseph Longworth
Gunnar Dittmar
An antigen microarray protocol for COVID-19 serological analysis
STAR Protocols
Antibody
Bioinformatics
Health Sciences
High Throughput Screening
Immunology
Proteomics
author_facet Joseph Longworth
Gunnar Dittmar
author_sort Joseph Longworth
title An antigen microarray protocol for COVID-19 serological analysis
title_short An antigen microarray protocol for COVID-19 serological analysis
title_full An antigen microarray protocol for COVID-19 serological analysis
title_fullStr An antigen microarray protocol for COVID-19 serological analysis
title_full_unstemmed An antigen microarray protocol for COVID-19 serological analysis
title_sort antigen microarray protocol for covid-19 serological analysis
publisher Elsevier
series STAR Protocols
issn 2666-1667
publishDate 2021-09-01
description Summary: The emergence of the coronavirus disease 2019 pandemic increased the interest in analysis of immunoglobulin responses. ELISA and lateral flow assays are widely used but are restricted by a single response value to an antigen or antigen pool. Here, we describe antigen microarrays, an alternative allowing simultaneous assessment of multiple interactions between antigens and the immunoglobulin content of patient sera. The technique requires minimal reagents and sample input and can be adapted to a wide variety of potential antigenic targets of interest.
topic Antibody
Bioinformatics
Health Sciences
High Throughput Screening
Immunology
Proteomics
url http://www.sciencedirect.com/science/article/pii/S2666166721005219
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