An antigen microarray protocol for COVID-19 serological analysis

An antigen microarray protocol for COVID-19 serological analysis

Summary: The emergence of the coronavirus disease 2019 pandemic increased the interest in analysis of immunoglobulin responses. ELISA and lateral flow assays are widely used but are restricted by a single response value to an antigen or antigen pool. Here, we describe antigen microarrays, an alterna...

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Bibliographic Details
Main Authors: Joseph Longworth, Gunnar Dittmar
Format: Article
Language:English
Published: Elsevier 2021-09-01
Series:STAR Protocols
Subjects:
Antibody
Bioinformatics
Health Sciences
High Throughput Screening
Immunology
Proteomics
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721005219
Description
Summary:Summary: The emergence of the coronavirus disease 2019 pandemic increased the interest in analysis of immunoglobulin responses. ELISA and lateral flow assays are widely used but are restricted by a single response value to an antigen or antigen pool. Here, we describe antigen microarrays, an alternative allowing simultaneous assessment of multiple interactions between antigens and the immunoglobulin content of patient sera. The technique requires minimal reagents and sample input and can be adapted to a wide variety of potential antigenic targets of interest.
ISSN:2666-1667

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