An Improved Method to Knock Out the asd Gene of Salmonella enterica Serovar Pullorum

An Improved Method to Knock Out the asd Gene of Salmonella enterica Serovar Pullorum

An asd-deleted (Δasd) mutant of Salmonella enterica serovar Pullorum (SP) was constructed using an improved method of gene knockout by combining the π-suicide plasmid system with the Red Disruption system. The asd gene was efficiently knocked out by the recombinant suicide vector, which replaced the...

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Main Authors: Shi-Zhong Geng, Xin-An Jiao, Zhi-Ming Pan, Xiao-Juan Chen, Xiao-Ming Zhang, Xiang Chen
Format: Article
Language:English
Published: Hindawi Limited 2009-01-01
Series:Journal of Biomedicine and Biotechnology
Online Access:http://dx.doi.org/10.1155/2009/646380
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spelling doaj-a49f9e81b934489ab30680f223a4db292020-11-25T00:03:59ZengHindawi LimitedJournal of Biomedicine and Biotechnology1110-72431110-72512009-01-01200910.1155/2009/646380646380An Improved Method to Knock Out the asd Gene of Salmonella enterica Serovar PullorumShi-Zhong Geng0Xin-An Jiao1Zhi-Ming Pan2Xiao-Juan Chen3Xiao-Ming Zhang4Xiang Chen5Jiangsu Key Laboratory of Zoonosis, Yangzhou University, Yangzhou, Jiangsu 225009, ChinaJiangsu Key Laboratory of Zoonosis, Yangzhou University, Yangzhou, Jiangsu 225009, ChinaJiangsu Key Laboratory of Zoonosis, Yangzhou University, Yangzhou, Jiangsu 225009, ChinaJiangsu Key Laboratory of Zoonosis, Yangzhou University, Yangzhou, Jiangsu 225009, ChinaJiangsu Key Laboratory of Zoonosis, Yangzhou University, Yangzhou, Jiangsu 225009, ChinaJiangsu Key Laboratory of Zoonosis, Yangzhou University, Yangzhou, Jiangsu 225009, ChinaAn asd-deleted (Δasd) mutant of Salmonella enterica serovar Pullorum (SP) was constructed using an improved method of gene knockout by combining the π-suicide plasmid system with the Red Disruption system. The asd gene was efficiently knocked out by the recombinant suicide vector, which replaced the asd gene with the CmR gene. Based on the balanced lethal host-vector system, the phenotype of the Δasd mutant was further defined. The improved method was simpler and more effective than previously reported conventional methods.http://dx.doi.org/10.1155/2009/646380
collection DOAJ
language English
format Article
sources DOAJ
author Shi-Zhong Geng
Xin-An Jiao
Zhi-Ming Pan
Xiao-Juan Chen
Xiao-Ming Zhang
Xiang Chen
spellingShingle Shi-Zhong Geng
Xin-An Jiao
Zhi-Ming Pan
Xiao-Juan Chen
Xiao-Ming Zhang
Xiang Chen
An Improved Method to Knock Out the asd Gene of Salmonella enterica Serovar Pullorum
Journal of Biomedicine and Biotechnology
author_facet Shi-Zhong Geng
Xin-An Jiao
Zhi-Ming Pan
Xiao-Juan Chen
Xiao-Ming Zhang
Xiang Chen
author_sort Shi-Zhong Geng
title An Improved Method to Knock Out the asd Gene of Salmonella enterica Serovar Pullorum
title_short An Improved Method to Knock Out the asd Gene of Salmonella enterica Serovar Pullorum
title_full An Improved Method to Knock Out the asd Gene of Salmonella enterica Serovar Pullorum
title_fullStr An Improved Method to Knock Out the asd Gene of Salmonella enterica Serovar Pullorum
title_full_unstemmed An Improved Method to Knock Out the asd Gene of Salmonella enterica Serovar Pullorum
title_sort improved method to knock out the asd gene of salmonella enterica serovar pullorum
publisher Hindawi Limited
series Journal of Biomedicine and Biotechnology
issn 1110-7243
1110-7251
publishDate 2009-01-01
description An asd-deleted (Δasd) mutant of Salmonella enterica serovar Pullorum (SP) was constructed using an improved method of gene knockout by combining the π-suicide plasmid system with the Red Disruption system. The asd gene was efficiently knocked out by the recombinant suicide vector, which replaced the asd gene with the CmR gene. Based on the balanced lethal host-vector system, the phenotype of the Δasd mutant was further defined. The improved method was simpler and more effective than previously reported conventional methods.
url http://dx.doi.org/10.1155/2009/646380
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