Investigations of the capability of Fusarium isolates from corn for biosynthesis of fusariotoxins

• Main Authors: Jajić Igor M., Bočarov-Stančić Aleksandra S., Bijelić Milorad B.
• Format: Article
• Language: English
• Published: Matica srpska 2007-01-01
• Series: Zbornik Matice Srpske za Prirodne Nauke
• Subjects: biosynthesis; corn grains; fusariotoxins; Fusarium spp
• Online Access: http://www.doiserbia.nb.rs/img/doi/0352-4906/2007/0352-49060713125J.pdf

The aim of this paper was to investigate the potential of zearalenone (ZEN) and type A trichothecenes (T-2 toxin and diacetoxyscirpenole - DAS) for biosynthesis by Fusarium spp. isolated from corn kernels contaminated by mycotoxins. The samples of corn kernels (2004 and 2005 harvest) originating in different regions of Bačka (Vojvodina, Serbia) were tested. Mycotoxicological investigations showed in most cases a significant contamination with deoxynivalenol (DON 200 - 2,460 μg/kg) and ZEA (520 1,680 μg/kg). Isolations and identifications of fusaria established only the presence of species F. verticillioides, after one month storage in freezer conditions, in fusariotoxin positive samples. The control cultures known as ZEA producers - F. graminearum GZ-LES, i.e. T-2 toxin and DAS producer - F. sporotrichioides KF-38/1/R were also tested. In vitro toxicological investigations of isolated fusaria were performed in liquid semisynthetic media (GPK or SPK), and on wet sterilized corn kernels, respectively. Under testing conditions, analyzed F. verticillioide and F. sporotrishioides isolates were not ZEA producers. Contrary to them, F. graminearum GZ-LES pure culture was very good producer of fusariotoxins; it biosynthesized max. 465,900 μg/kg DON, and 4,416 μg/kg ZEA, respectively. Cultivation conditions influenced a great deal of T-2 toxin production under laboratory conditions. In most cases, higher yields were obtained during the cultivation of F. verticillioides in liquid glucose medium (80-240 μg/L). Contrary to the control strain F. sporotrichioides KF-38/1/R that under the same conditions synthesized, besides T-2 toxin (4.000 μg/L) and DAS (240 μg/L), isolates of F. verticillioides from corn grain did not show that ability.