RNA-Targeting CRISPR–Cas Systems and Their Applications

RNA-Targeting CRISPR–Cas Systems and Their Applications

Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)−CRISPR-associated (Cas) systems have revolutionized modern molecular biology. Numerous types of these systems have been discovered to date. Many CRISPR−Cas systems have been used as a backbone for the development...

Full description

Bibliographic Details
Main Authors: Michal Burmistrz, Kamil Krakowski, Agata Krawczyk-Balska
Format: Article
Language:English
Published: MDPI AG 2020-02-01
Series:International Journal of Molecular Sciences
Subjects:
crispr–cas
rna
cas9
cas13
cmr
csm
Online Access:https://www.mdpi.com/1422-0067/21/3/1122
id doaj-a436e3996ebd4370a769ee8b1f1ac34f
record_format Article
spelling doaj-a436e3996ebd4370a769ee8b1f1ac34f2020-11-25T01:30:42ZengMDPI AGInternational Journal of Molecular Sciences1422-00672020-02-01213112210.3390/ijms21031122ijms21031122RNA-Targeting CRISPR–Cas Systems and Their ApplicationsMichal Burmistrz0Kamil Krakowski1Agata Krawczyk-Balska2Department of Molecular Microbiology, Biological and Chemical Research Centre, Faculty of Biology, University of Warsaw, 02-089 Warsaw, PolandDepartment of Molecular Microbiology, Biological and Chemical Research Centre, Faculty of Biology, University of Warsaw, 02-089 Warsaw, PolandDepartment of Molecular Microbiology, Biological and Chemical Research Centre, Faculty of Biology, University of Warsaw, 02-089 Warsaw, PolandClustered Regularly Interspaced Short Palindromic Repeats (CRISPR)−CRISPR-associated (Cas) systems have revolutionized modern molecular biology. Numerous types of these systems have been discovered to date. Many CRISPR−Cas systems have been used as a backbone for the development of potent research tools, with Cas9 being the most widespread. While most of the utilized systems are DNA-targeting, recently more and more attention is being gained by those that target RNA. Their ability to specifically recognize a given RNA sequence in an easily programmable way makes them ideal candidates for developing new research tools. In this review we summarize current knowledge on CRISPR−Cas systems which have been shown to target RNA molecules, that is type III (Csm/Cmr), type VI (Cas13), and type II (Cas9). We also present a list of available technologies based on these systems.https://www.mdpi.com/1422-0067/21/3/1122crispr–casrnacas9cas13cmrcsm
collection DOAJ
language English
format Article
sources DOAJ
author Michal Burmistrz
Kamil Krakowski
Agata Krawczyk-Balska
spellingShingle Michal Burmistrz
Kamil Krakowski
Agata Krawczyk-Balska
RNA-Targeting CRISPR–Cas Systems and Their Applications
International Journal of Molecular Sciences
crispr–cas
rna
cas9
cas13
cmr
csm
author_facet Michal Burmistrz
Kamil Krakowski
Agata Krawczyk-Balska
author_sort Michal Burmistrz
title RNA-Targeting CRISPR–Cas Systems and Their Applications
title_short RNA-Targeting CRISPR–Cas Systems and Their Applications
title_full RNA-Targeting CRISPR–Cas Systems and Their Applications
title_fullStr RNA-Targeting CRISPR–Cas Systems and Their Applications
title_full_unstemmed RNA-Targeting CRISPR–Cas Systems and Their Applications
title_sort rna-targeting crispr–cas systems and their applications
publisher MDPI AG
series International Journal of Molecular Sciences
issn 1422-0067
publishDate 2020-02-01
description Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)−CRISPR-associated (Cas) systems have revolutionized modern molecular biology. Numerous types of these systems have been discovered to date. Many CRISPR−Cas systems have been used as a backbone for the development of potent research tools, with Cas9 being the most widespread. While most of the utilized systems are DNA-targeting, recently more and more attention is being gained by those that target RNA. Their ability to specifically recognize a given RNA sequence in an easily programmable way makes them ideal candidates for developing new research tools. In this review we summarize current knowledge on CRISPR−Cas systems which have been shown to target RNA molecules, that is type III (Csm/Cmr), type VI (Cas13), and type II (Cas9). We also present a list of available technologies based on these systems.
topic crispr–cas
rna
cas9
cas13
cmr
csm
url https://www.mdpi.com/1422-0067/21/3/1122
work_keys_str_mv AT michalburmistrz rnatargetingcrisprcassystemsandtheirapplications
AT kamilkrakowski rnatargetingcrisprcassystemsandtheirapplications
AT agatakrawczykbalska rnatargetingcrisprcassystemsandtheirapplications
_version_ 1725090467775774720

Similar Items